Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7061 | 3' | -61 | NC_001876.1 | + | 24901 | 0.66 | 0.310645 |
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Target: 5'- cUUgCUCGGCUGCAGCCCCgcugucugUGGcGG-Ca -3' miRNA: 3'- cGAgGAGUUGACGUCGGGG--------GCC-CCaG- -5' |
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| 7061 | 3' | -61 | NC_001876.1 | + | 8933 | 0.67 | 0.267091 |
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Target: 5'- uCUCCUgggUAACgGCAcGCCCguguucUCGGGGUCg -3' miRNA: 3'- cGAGGA---GUUGaCGU-CGGG------GGCCCCAG- -5' |
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| 7061 | 3' | -61 | NC_001876.1 | + | 13852 | 0.68 | 0.22267 |
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Target: 5'- gGgUCUUCGcccacgugauaACUGC-GCCCCuUGGGGUCc -3' miRNA: 3'- -CgAGGAGU-----------UGACGuCGGGG-GCCCCAG- -5' |
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| 7061 | 3' | -61 | NC_001876.1 | + | 11844 | 0.69 | 0.184194 |
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Target: 5'- cGCUCgUguACUggcgcgcGCAcGCCCCagaGGGGUCg -3' miRNA: 3'- -CGAGgAguUGA-------CGU-CGGGGg--CCCCAG- -5' |
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| 7061 | 3' | -61 | NC_001876.1 | + | 10265 | 0.7 | 0.147168 |
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Target: 5'- gGUUCCUCcccGCUGCGGUcgcggaauaaguucUCCCGGGGa- -3' miRNA: 3'- -CGAGGAGu--UGACGUCG--------------GGGGCCCCag -5' |
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| 7061 | 3' | -61 | NC_001876.1 | + | 23598 | 0.72 | 0.109073 |
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Target: 5'- gGCUCCUCGGCUucugGCAGCgCCUcagcuuccgguuCGGcGGUCg -3' miRNA: 3'- -CGAGGAGUUGA----CGUCG-GGG------------GCC-CCAG- -5' |
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| 7061 | 3' | -61 | NC_001876.1 | + | 26699 | 1.11 | 0.000095 |
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Target: 5'- gGCUCCUCAACUGCAGCCCCCGGGGUCg -3' miRNA: 3'- -CGAGGAGUUGACGUCGGGGGCCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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