Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7061 | 5' | -51.1 | NC_001876.1 | + | 11020 | 0.66 | 0.794887 |
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Target: 5'- -cGACCGCGGCGGCcGCuUUGccgGGg- -3' miRNA: 3'- gaCUGGUGUUGUCGuCGuAACa--CCac -5' |
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| 7061 | 5' | -51.1 | NC_001876.1 | + | 15566 | 0.67 | 0.728783 |
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Target: 5'- -cGGCCACAGcCGGUAGCAgc-UGGg- -3' miRNA: 3'- gaCUGGUGUU-GUCGUCGUaacACCac -5' |
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| 7061 | 5' | -51.1 | NC_001876.1 | + | 17831 | 0.69 | 0.611068 |
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Target: 5'- -gGACCAUuGCGGCAGCAUcGacggGGUa -3' miRNA: 3'- gaCUGGUGuUGUCGUCGUAaCa---CCAc -5' |
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| 7061 | 5' | -51.1 | NC_001876.1 | + | 12554 | 0.7 | 0.563856 |
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Target: 5'- -aGGuCCGCGGCaccgGGCGGCGgcgGUGGUGu -3' miRNA: 3'- gaCU-GGUGUUG----UCGUCGUaa-CACCAC- -5' |
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| 7061 | 5' | -51.1 | NC_001876.1 | + | 11587 | 0.7 | 0.529083 |
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Target: 5'- cCUGAgCAC-ACAGcCAGCg--GUGGUGg -3' miRNA: 3'- -GACUgGUGuUGUC-GUCGuaaCACCAC- -5' |
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| 7061 | 5' | -51.1 | NC_001876.1 | + | 26738 | 1.09 | 0.001289 |
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Target: 5'- aCUGACCACAACAGCAGCAUUGUGGUGc -3' miRNA: 3'- -GACUGGUGUUGUCGUCGUAACACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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