Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7062 | 3' | -47.8 | NC_001876.1 | + | 30257 | 0.66 | 0.919606 |
Target: 5'- cACUUcCAGCUgG--UUGGuGACGGGGUc -3' miRNA: 3'- uUGAA-GUCGAgUuaAACC-CUGUUCCG- -5' |
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7062 | 3' | -47.8 | NC_001876.1 | + | 30705 | 0.67 | 0.864264 |
Target: 5'- aGACUcccUCGGCUUucgUUGaGGAUggGGUa -3' miRNA: 3'- -UUGA---AGUCGAGuuaAAC-CCUGuuCCG- -5' |
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7062 | 3' | -47.8 | NC_001876.1 | + | 29353 | 0.7 | 0.71446 |
Target: 5'- ----cCAGCggauccgCAagGUUUGGGACAAGGUu -3' miRNA: 3'- uugaaGUCGa------GU--UAAACCCUGUUCCG- -5' |
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7062 | 3' | -47.8 | NC_001876.1 | + | 30161 | 1.1 | 0.002404 |
Target: 5'- aAACUUCAGCUCAAUUUGGGACAAGGCc -3' miRNA: 3'- -UUGAAGUCGAGUUAAACCCUGUUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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