Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7063 | 5' | -49.8 | NC_001876.1 | + | 11222 | 0.66 | 0.859412 |
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Target: 5'- aGGAUUGGAAgGGGCGGggcccagGCUu-- -3' miRNA: 3'- -CCUGGCCUUgUCCGUCauua---CGAuua -5' |
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| 7063 | 5' | -49.8 | NC_001876.1 | + | 34262 | 0.66 | 0.850295 |
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Target: 5'- cGGGgCGGGGCaaaGGGCAGg---GCUAc- -3' miRNA: 3'- -CCUgGCCUUG---UCCGUCauuaCGAUua -5' |
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| 7063 | 5' | -49.8 | NC_001876.1 | + | 30369 | 0.67 | 0.800878 |
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Target: 5'- uGACCauguuaGGAGC-GGUAGUGAUGCg--- -3' miRNA: 3'- cCUGG------CCUUGuCCGUCAUUACGauua -5' |
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| 7063 | 5' | -49.8 | NC_001876.1 | + | 12547 | 0.68 | 0.746258 |
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Target: 5'- cGGcACCGGGcgGC-GGCGGUGGUGUg--- -3' miRNA: 3'- -CC-UGGCCU--UGuCCGUCAUUACGauua -5' |
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| 7063 | 5' | -49.8 | NC_001876.1 | + | 12946 | 0.69 | 0.688216 |
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Target: 5'- -cGCCGGcuuAugAGGCGGUGGUGgCUGGg -3' miRNA: 3'- ccUGGCC---UugUCCGUCAUUAC-GAUUa -5' |
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| 7063 | 5' | -49.8 | NC_001876.1 | + | 9013 | 0.76 | 0.303055 |
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Target: 5'- cGGGCUGGGGCAGGCug-AGUGCg--- -3' miRNA: 3'- -CCUGGCCUUGUCCGucaUUACGauua -5' |
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| 7063 | 5' | -49.8 | NC_001876.1 | + | 30347 | 1.09 | 0.001733 |
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Target: 5'- uGGACCGGAACAGGCAGUAAUGCUAAUg -3' miRNA: 3'- -CCUGGCCUUGUCCGUCAUUACGAUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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