Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7103 | 3' | -56.8 | NC_001895.1 | + | 2897 | 0.66 | 0.510581 |
Target: 5'- aGCUGgaaacagCGCGACG-GCUGGGACaGCg-- -3' miRNA: 3'- -CGGCa------GUGCUGCgCGACUCUG-CGacu -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 62 | 0.66 | 0.507389 |
Target: 5'- aGCgCGUCGUGACGgcacuuagucugccCGUUGAGGCGUUGu -3' miRNA: 3'- -CG-GCAGUGCUGC--------------GCGACUCUGCGACu -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 29824 | 0.66 | 0.507389 |
Target: 5'- cGUCGUUcuggugauaugaauAUGACGCGCgccGAGAUGCg-- -3' miRNA: 3'- -CGGCAG--------------UGCUGCGCGa--CUCUGCGacu -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 30165 | 0.66 | 0.489465 |
Target: 5'- gGCC-UCAgCGAUGgGaacuUGAGGCGCUGGc -3' miRNA: 3'- -CGGcAGU-GCUGCgCg---ACUCUGCGACU- -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 13596 | 0.67 | 0.458587 |
Target: 5'- cUCGUCGCcuGACGCaCUGAauACGCUGAa -3' miRNA: 3'- cGGCAGUG--CUGCGcGACUc-UGCGACU- -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 6430 | 0.67 | 0.448526 |
Target: 5'- aGCUGUgGCGGgauaUGCGCUG-GGCGgUGGc -3' miRNA: 3'- -CGGCAgUGCU----GCGCGACuCUGCgACU- -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 20290 | 0.67 | 0.448526 |
Target: 5'- aCCGUCAgcgUGAUGCGCUGGuGCGUg-- -3' miRNA: 3'- cGGCAGU---GCUGCGCGACUcUGCGacu -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 22955 | 0.68 | 0.372758 |
Target: 5'- uGCCGguuCGugGCGCUGuccuGACGgUGu -3' miRNA: 3'- -CGGCaguGCugCGCGACu---CUGCgACu -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 18476 | 0.69 | 0.338277 |
Target: 5'- aCCGUCGCGGCGaaucaGCguaGuGACGcCUGAc -3' miRNA: 3'- cGGCAGUGCUGCg----CGa--CuCUGC-GACU- -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 5348 | 0.7 | 0.306173 |
Target: 5'- uGCCGUgggugaACGGCGUGCUG-GA-GCUGGg -3' miRNA: 3'- -CGGCAg-----UGCUGCGCGACuCUgCGACU- -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 18524 | 0.77 | 0.102425 |
Target: 5'- gGCCGaCACGAUG-GCUGAGGCGCa-- -3' miRNA: 3'- -CGGCaGUGCUGCgCGACUCUGCGacu -5' |
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7103 | 3' | -56.8 | NC_001895.1 | + | 111 | 1.11 | 0.000311 |
Target: 5'- uGCCGUCACGACGCGCUGAGACGCUGAa -3' miRNA: 3'- -CGGCAGUGCUGCGCGACUCUGCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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