Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7103 | 5' | -57.5 | NC_001895.1 | + | 18229 | 0.66 | 0.382184 |
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Target: 5'- --cCAGUACCACCGcCAcCGCGUAu- -3' miRNA: 3'- uuaGUCGUGGUGGCaGUcGCGCGUca -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 19629 | 0.67 | 0.329301 |
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Target: 5'- cGGUCAGCucGgUGCCGUCAuGCGCgGCAGc -3' miRNA: 3'- -UUAGUCG--UgGUGGCAGU-CGCG-CGUCa -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 1686 | 0.68 | 0.32103 |
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Target: 5'- --cCGGcCACCACCGgCGGUGC-CAGUa -3' miRNA: 3'- uuaGUC-GUGGUGGCaGUCGCGcGUCA- -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 17869 | 0.68 | 0.32103 |
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Target: 5'- --cCGGuCACCGuuGUCAuGCGCGUGGa -3' miRNA: 3'- uuaGUC-GUGGUggCAGU-CGCGCGUCa -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 8535 | 0.68 | 0.297156 |
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Target: 5'- -uUCAGCACCgugacgGCaucaGUCAGCgcccuGCGCAGg -3' miRNA: 3'- uuAGUCGUGG------UGg---CAGUCG-----CGCGUCa -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 29734 | 0.69 | 0.260471 |
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Target: 5'- uGGUCGGCAUuuacgCGCCGcaucUCGGCGCGCGu- -3' miRNA: 3'- -UUAGUCGUG-----GUGGC----AGUCGCGCGUca -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 13677 | 0.69 | 0.253592 |
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Target: 5'- --cCAGCGCCGCCGccagcucguUCAGCGUauuCAGUg -3' miRNA: 3'- uuaGUCGUGGUGGC---------AGUCGCGc--GUCA- -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 4369 | 0.72 | 0.167453 |
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Target: 5'- -cUCGGUACCACCGgCAGUGUcgGUAGUg -3' miRNA: 3'- uuAGUCGUGGUGGCaGUCGCG--CGUCA- -5' |
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| 7103 | 5' | -57.5 | NC_001895.1 | + | 77 | 1.05 | 0.000448 |
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Target: 5'- cAAUCAGCACCACCGUCAGCGCGCAGUg -3' miRNA: 3'- -UUAGUCGUGGUGGCAGUCGCGCGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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