Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7105 | 3' | -53.5 | NC_001895.1 | + | 9282 | 0.66 | 0.655379 |
Target: 5'- cGCGUccccugcuCGGCuuUACCGGUGAGGa- -3' miRNA: 3'- uCGCAau------GUCGugGUGGCCAUUCCag -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 21713 | 0.66 | 0.620478 |
Target: 5'- gGGCugaaaGCGGCAUuugCGCCGGUGGGGg- -3' miRNA: 3'- -UCGcaa--UGUCGUG---GUGGCCAUUCCag -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 22532 | 0.67 | 0.597248 |
Target: 5'- gAGCGUggucaucguCAGCuuGCCGCCGGUcAGuUCg -3' miRNA: 3'- -UCGCAau-------GUCG--UGGUGGCCAuUCcAG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 10433 | 0.67 | 0.597248 |
Target: 5'- cGCG--GCAGCgACCGgCGuUAAGGUCg -3' miRNA: 3'- uCGCaaUGUCG-UGGUgGCcAUUCCAG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 23715 | 0.67 | 0.596089 |
Target: 5'- gGGCGUUGCggaguucucuaucAGCcugGCUACCGGUcGGG-Ca -3' miRNA: 3'- -UCGCAAUG-------------UCG---UGGUGGCCAuUCCaG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 12001 | 0.67 | 0.562676 |
Target: 5'- aAGCGUauaucACGGCACagCACCGGcUGGGG-Cg -3' miRNA: 3'- -UCGCAa----UGUCGUG--GUGGCC-AUUCCaG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 29393 | 0.68 | 0.528664 |
Target: 5'- cGCGauauaccccGCAGCACCGCCcuGGuUAAGGUg -3' miRNA: 3'- uCGCaa-------UGUCGUGGUGG--CC-AUUCCAg -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 3962 | 0.68 | 0.506416 |
Target: 5'- cAGCGccGCAGCaaaGCCACCGGc--GGUg -3' miRNA: 3'- -UCGCaaUGUCG---UGGUGGCCauuCCAg -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 5540 | 0.69 | 0.483496 |
Target: 5'- uAGCGcUUGCGGCcaugcgcgcucacGCUGCCGGUGA-GUCu -3' miRNA: 3'- -UCGC-AAUGUCG-------------UGGUGGCCAUUcCAG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 11889 | 0.69 | 0.473827 |
Target: 5'- gGGCGUUAC-GCACCACagccagcAGGUCu -3' miRNA: 3'- -UCGCAAUGuCGUGGUGgccau--UCCAG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 1403 | 0.74 | 0.252585 |
Target: 5'- cAGCGgacGCAGCGCCACCuaUGAGGcCa -3' miRNA: 3'- -UCGCaa-UGUCGUGGUGGccAUUCCaG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 1749 | 0.76 | 0.175483 |
Target: 5'- uGCGUggUACuGGCACCGCCGGUGGuGGcCg -3' miRNA: 3'- uCGCA--AUG-UCGUGGUGGCCAUU-CCaG- -5' |
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7105 | 3' | -53.5 | NC_001895.1 | + | 911 | 1.11 | 0.000554 |
Target: 5'- aAGCGUUACAGCACCACCGGUAAGGUCa -3' miRNA: 3'- -UCGCAAUGUCGUGGUGGCCAUUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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