Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7105 | 5' | -47.6 | NC_001895.1 | + | 28679 | 0.66 | 0.950592 |
Target: 5'- cAGACgggCAauGGCAGAAUAUGCCUGc -3' miRNA: 3'- cUUUGga-GUggCCGUUUUAUAUGGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 19117 | 0.66 | 0.945551 |
Target: 5'- -cGACgaUACCGGCGAAAcgGUGgCCGu -3' miRNA: 3'- cuUUGgaGUGGCCGUUUUa-UAUgGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 29883 | 0.66 | 0.945551 |
Target: 5'- gGAAAgCUCACCGGUGGug-AUACUUc -3' miRNA: 3'- -CUUUgGAGUGGCCGUUuuaUAUGGGc -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 10399 | 0.66 | 0.934488 |
Target: 5'- -cGugCagACCGGCGGcAUGUGCaCCGa -3' miRNA: 3'- cuUugGagUGGCCGUUuUAUAUG-GGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 6879 | 0.67 | 0.922098 |
Target: 5'- cGAGACaaaacCACCGaGCAAcAUGcGCCCGa -3' miRNA: 3'- -CUUUGga---GUGGC-CGUUuUAUaUGGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 26509 | 0.67 | 0.915403 |
Target: 5'- ---uCCUCGCUGGCAGGAUAcAUgUGa -3' miRNA: 3'- cuuuGGAGUGGCCGUUUUAUaUGgGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 8997 | 0.67 | 0.915403 |
Target: 5'- cGAAACgUCugCGGCAGAGUcaGCagCGu -3' miRNA: 3'- -CUUUGgAGugGCCGUUUUAuaUGg-GC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 13015 | 0.67 | 0.908378 |
Target: 5'- aGAAACCgcccaccuCCGGCGGAAUAaccaGCUCGg -3' miRNA: 3'- -CUUUGGagu-----GGCCGUUUUAUa---UGGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 23164 | 0.69 | 0.84846 |
Target: 5'- aGGAGCCUUcCCGGCGGGAauugagcguCCCGc -3' miRNA: 3'- -CUUUGGAGuGGCCGUUUUauau-----GGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 12709 | 0.69 | 0.821145 |
Target: 5'- uAAGCCacacCACCGGCGAAcagAUAgCCGg -3' miRNA: 3'- cUUUGGa---GUGGCCGUUUua-UAUgGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 2015 | 0.7 | 0.789807 |
Target: 5'- aGAAGCC-CACCGGCAAGGgcguUAUGaCUGa -3' miRNA: 3'- -CUUUGGaGUGGCCGUUUU----AUAUgGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 23430 | 0.7 | 0.783314 |
Target: 5'- uGGAGCCUaCACCgguguaacGGCAAAAUGgcuacacacuaaagACCCGa -3' miRNA: 3'- -CUUUGGA-GUGG--------CCGUUUUAUa-------------UGGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 28050 | 0.71 | 0.710464 |
Target: 5'- --uGCCgcaggCACCGGUAAGAUGUuuaCCGg -3' miRNA: 3'- cuuUGGa----GUGGCCGUUUUAUAug-GGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 5845 | 0.72 | 0.662751 |
Target: 5'- cGGAGCgUgaCACCGGCAAAacGUGgacGCCCGa -3' miRNA: 3'- -CUUUGgA--GUGGCCGUUU--UAUa--UGGGC- -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 23661 | 0.73 | 0.590405 |
Target: 5'- -cAGCCUCACCGGCc--AUGUAUUCa -3' miRNA: 3'- cuUUGGAGUGGCCGuuuUAUAUGGGc -5' |
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7105 | 5' | -47.6 | NC_001895.1 | + | 876 | 1.09 | 0.003469 |
Target: 5'- gGAAACCUCACCGGCAAAAUAUACCCGc -3' miRNA: 3'- -CUUUGGAGUGGCCGUUUUAUAUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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