Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7107 | 3' | -50.1 | NC_001895.1 | + | 19727 | 0.66 | 0.862908 |
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Target: 5'- uGUgGCAGUGauauuucacUGGCCGcCAUCAGAAc- -3' miRNA: 3'- -CGgUGUCGU---------ACUGGCaGUAGUCUUac -5' |
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| 7107 | 3' | -50.1 | NC_001895.1 | + | 908 | 0.67 | 0.805448 |
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Target: 5'- cGUUACAGCAccACCGguaaggUCAUCAGAcUGg -3' miRNA: 3'- -CGGUGUCGUacUGGC------AGUAGUCUuAC- -5' |
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| 7107 | 3' | -50.1 | NC_001895.1 | + | 22537 | 0.68 | 0.751656 |
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Target: 5'- aGCCugAGCGUGGUCaUCGUCAGcuUGc -3' miRNA: 3'- -CGGugUCGUACUGGcAGUAGUCuuAC- -5' |
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| 7107 | 3' | -50.1 | NC_001895.1 | + | 4056 | 0.69 | 0.694323 |
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Target: 5'- cGCCGguCAGCAgu-UCGUCAUCAGGGc- -3' miRNA: 3'- -CGGU--GUCGUacuGGCAGUAGUCUUac -5' |
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| 7107 | 3' | -50.1 | NC_001895.1 | + | 14261 | 0.71 | 0.587808 |
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Target: 5'- uGCUAacaguCAGCGUGuauuCCGUUuUCAGAAUGg -3' miRNA: 3'- -CGGU-----GUCGUACu---GGCAGuAGUCUUAC- -5' |
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| 7107 | 3' | -50.1 | NC_001895.1 | + | 20713 | 0.71 | 0.576033 |
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Target: 5'- cGCCAuguuCAGCGUGACCGgCGUUgccgccuGAAUGg -3' miRNA: 3'- -CGGU----GUCGUACUGGCaGUAGu------CUUAC- -5' |
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| 7107 | 3' | -50.1 | NC_001895.1 | + | 22183 | 0.71 | 0.564313 |
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Target: 5'- -aCACAGCuUGACCGcCAguuaCAGGAUGc -3' miRNA: 3'- cgGUGUCGuACUGGCaGUa---GUCUUAC- -5' |
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| 7107 | 3' | -50.1 | NC_001895.1 | + | 5456 | 1.11 | 0.001219 |
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Target: 5'- cGCCACAGCAUGACCGUCAUCAGAAUGu -3' miRNA: 3'- -CGGUGUCGUACUGGCAGUAGUCUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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