Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7109 | 3' | -54.2 | NC_001895.1 | + | 1146 | 0.66 | 0.652532 |
Target: 5'- uGCCUccauuUUCGggcCGCUGGCggUCAUuguuuuuGCCGCa -3' miRNA: 3'- -CGGA-----AAGC---GUGACCGuaGGUG-------UGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 28926 | 0.66 | 0.630697 |
Target: 5'- uGCCg---GUAUUGaGCGUUCGcCGCCGCa -3' miRNA: 3'- -CGGaaagCGUGAC-CGUAGGU-GUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 5948 | 0.66 | 0.607719 |
Target: 5'- aCCUgacacUGCACcGGCGUCC--ACCGCc -3' miRNA: 3'- cGGAaa---GCGUGaCCGUAGGugUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 20366 | 0.66 | 0.607719 |
Target: 5'- uGCCUguugCGCACUGaCA--CGCACCaGCg -3' miRNA: 3'- -CGGAaa--GCGUGACcGUagGUGUGG-CG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 21584 | 0.66 | 0.607719 |
Target: 5'- cGCCUgcugcCGCGaugaUGGCAUUgAUGCCGg -3' miRNA: 3'- -CGGAaa---GCGUg---ACCGUAGgUGUGGCg -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 8378 | 0.67 | 0.573449 |
Target: 5'- aGCUUgagaaCGCGCUGGCA--CGCugUGCc -3' miRNA: 3'- -CGGAaa---GCGUGACCGUagGUGugGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 12754 | 0.67 | 0.562118 |
Target: 5'- uGCCgg-UGCagcaaaGCUGGCAgcggcaaCCGCGCCGg -3' miRNA: 3'- -CGGaaaGCG------UGACCGUa------GGUGUGGCg -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 6447 | 0.67 | 0.562118 |
Target: 5'- cGCUgggcgguggCGCGCauccagGGCAagCCGCGCUGCa -3' miRNA: 3'- -CGGaaa------GCGUGa-----CCGUa-GGUGUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 14133 | 0.67 | 0.562118 |
Target: 5'- gGUCUUUUGUugUGGUAcUCugGCgGCu -3' miRNA: 3'- -CGGAAAGCGugACCGUaGGugUGgCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 10349 | 0.67 | 0.555349 |
Target: 5'- ---aUUCGCACUGGCAUuaucgucgaaaccgaCCuuaACGCCGg -3' miRNA: 3'- cggaAAGCGUGACCGUA---------------GG---UGUGGCg -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 23658 | 0.67 | 0.539649 |
Target: 5'- uGCCacgaaaGCGCaGGcCAUgCGCGCCGCu -3' miRNA: 3'- -CGGaaag--CGUGaCC-GUAgGUGUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 19014 | 0.68 | 0.517493 |
Target: 5'- aCCgg-UGCGCUGccguuCAUCCccGCACCGCg -3' miRNA: 3'- cGGaaaGCGUGACc----GUAGG--UGUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 5700 | 0.68 | 0.517493 |
Target: 5'- cGCCgg-UGCGCUGGCG--CAC-CUGCa -3' miRNA: 3'- -CGGaaaGCGUGACCGUagGUGuGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 18068 | 0.68 | 0.493553 |
Target: 5'- gGCUg-UUGCACUGGCAUCagucugucagaaGCugCGUg -3' miRNA: 3'- -CGGaaAGCGUGACCGUAGg-----------UGugGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 5747 | 0.68 | 0.484973 |
Target: 5'- cGCCag-CGCACcggcGGCAUCaC-CACCGUc -3' miRNA: 3'- -CGGaaaGCGUGa---CCGUAG-GuGUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 13411 | 0.69 | 0.463844 |
Target: 5'- gGCCuUUUCGCGCuguggUGGCGUCCuguGCgGUa -3' miRNA: 3'- -CGG-AAAGCGUG-----ACCGUAGGug-UGgCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 2971 | 0.69 | 0.442187 |
Target: 5'- cGCUgc-UGUACUGGCAgggguuuUCCGUGCCGCa -3' miRNA: 3'- -CGGaaaGCGUGACCGU-------AGGUGUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 28160 | 0.69 | 0.440154 |
Target: 5'- gGUCUUUCGCGCccgacggccucgcgUGGCGcguUCCAUGCauaaGCa -3' miRNA: 3'- -CGGAAAGCGUG--------------ACCGU---AGGUGUGg---CG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 28764 | 0.71 | 0.34844 |
Target: 5'- uGCCaUUCGUgaUGuGCGUgCACGCCGCc -3' miRNA: 3'- -CGGaAAGCGugAC-CGUAgGUGUGGCG- -5' |
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7109 | 3' | -54.2 | NC_001895.1 | + | 2594 | 0.73 | 0.25917 |
Target: 5'- aGUCUUUCgacuaucagcuGCACUGGUAUCgugccgggcuugaGCACCGCa -3' miRNA: 3'- -CGGAAAG-----------CGUGACCGUAGg------------UGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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