Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7110 | 3' | -56.9 | NC_001895.1 | + | 29734 | 0.66 | 0.503043 |
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Target: 5'- uGGUCggCAuuuaCGCGCCgcauCUCGGcGCGCg -3' miRNA: 3'- gCCAGa-GUug--GUGCGG----GAGUCaCGCG- -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 10410 | 0.66 | 0.499863 |
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Target: 5'- uCGGUUUCGACgauaAUGCCagugcgaaucauguUgcgCAGUGCGCg -3' miRNA: 3'- -GCCAGAGUUGg---UGCGG--------------Ga--GUCACGCG- -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 9024 | 0.66 | 0.492476 |
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Target: 5'- uCGGcaCUCAgucGCCgACGCCCUgaCGGUGacaGCg -3' miRNA: 3'- -GCCa-GAGU---UGG-UGCGGGA--GUCACg--CG- -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 28161 | 0.66 | 0.492476 |
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Target: 5'- gGGUCUUucgcGCCcgACGgCCUCGcGUGgCGCg -3' miRNA: 3'- gCCAGAGu---UGG--UGCgGGAGU-CAC-GCG- -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 26674 | 0.67 | 0.441294 |
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Target: 5'- uGGugcauUCUCAAa-ACGCCgUCAGguugGCGCa -3' miRNA: 3'- gCC-----AGAGUUggUGCGGgAGUCa---CGCG- -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 3890 | 0.67 | 0.441294 |
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Target: 5'- gCGGuuuccaUCUCAGCgagGCGCUgCUCAGUGgCGCu -3' miRNA: 3'- -GCC------AGAGUUGg--UGCGG-GAGUCAC-GCG- -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 23596 | 0.67 | 0.431421 |
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Target: 5'- cCGGUUUCGcuuUCGgGUgCUcCAGUGCGCg -3' miRNA: 3'- -GCCAGAGUu--GGUgCGgGA-GUCACGCG- -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 18566 | 0.69 | 0.307965 |
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Target: 5'- -cGUgUCGGCCACGaCCUgcgCGGUGCGg -3' miRNA: 3'- gcCAgAGUUGGUGC-GGGa--GUCACGCg -5' |
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| 7110 | 3' | -56.9 | NC_001895.1 | + | 6621 | 1.11 | 0.000264 |
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Target: 5'- aCGGUCUCAACCACGCCCUCAGUGCGCc -3' miRNA: 3'- -GCCAGAGUUGGUGCGGGAGUCACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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