Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7111 | 3' | -55.4 | NC_001895.1 | + | 5854 | 0.66 | 0.538519 |
Target: 5'- -aCACCG-GCAaaACGUGgaCGCCCGAa-- -3' miRNA: 3'- ugGUGGCuCGU--UGUAC--GCGGGCUauu -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 25685 | 0.66 | 0.52731 |
Target: 5'- uCCugaguCCGGGCAAauUGCGCCCGc--- -3' miRNA: 3'- uGGu----GGCUCGUUguACGCGGGCuauu -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 5697 | 0.66 | 0.509562 |
Target: 5'- uGCCGCCGGugcgcuggcgcaccuGCAACGUGCgacacaGCUCGAc-- -3' miRNA: 3'- -UGGUGGCU---------------CGUUGUACG------CGGGCUauu -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 3823 | 0.68 | 0.421145 |
Target: 5'- cGCCACUGAGCAGCGccuCGCUgaGAUGGa -3' miRNA: 3'- -UGGUGGCUCGUUGUac-GCGGg-CUAUU- -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 28285 | 0.68 | 0.421145 |
Target: 5'- -gCACCGGGCuuuuauUGCGCCgGAUGu -3' miRNA: 3'- ugGUGGCUCGuugu--ACGCGGgCUAUu -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 26463 | 0.68 | 0.373105 |
Target: 5'- aACaGCC-AGCAGCuAUGCGCCgGAUAAu -3' miRNA: 3'- -UGgUGGcUCGUUG-UACGCGGgCUAUU- -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 8841 | 0.69 | 0.346123 |
Target: 5'- cGCCGCCgGAGCcgguCAccCGCCCGAUGGa -3' miRNA: 3'- -UGGUGG-CUCGuu--GUacGCGGGCUAUU- -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 10391 | 0.7 | 0.320568 |
Target: 5'- cGCUGCCGcgugcagaccGGCGGCAUGUGCaCCGAc-- -3' miRNA: 3'- -UGGUGGC----------UCGUUGUACGCG-GGCUauu -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 9341 | 0.7 | 0.288733 |
Target: 5'- cACCgguaaaGCCGAGCAGgGgacGCGCCgGAUAAu -3' miRNA: 3'- -UGG------UGGCUCGUUgUa--CGCGGgCUAUU- -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 1771 | 0.71 | 0.252501 |
Target: 5'- -aCACCGuGGCGACA-GCGCCgGAUGc -3' miRNA: 3'- ugGUGGC-UCGUUGUaCGCGGgCUAUu -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 8832 | 0.71 | 0.245719 |
Target: 5'- cUCACUGAcCAGCGUGCGCUCGGUc- -3' miRNA: 3'- uGGUGGCUcGUUGUACGCGGGCUAuu -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 3976 | 0.76 | 0.116832 |
Target: 5'- aGCCACCGgcGGUGGCGUGaCGCCCuGAUGAc -3' miRNA: 3'- -UGGUGGC--UCGUUGUAC-GCGGG-CUAUU- -5' |
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7111 | 3' | -55.4 | NC_001895.1 | + | 6871 | 1.06 | 0.000684 |
Target: 5'- aACCACCGAGCAACAUGCGCCCGAUAAa -3' miRNA: 3'- -UGGUGGCUCGUUGUACGCGGGCUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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