Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7114 | 3' | -59 | NC_001895.1 | + | 5977 | 0.68 | 0.306467 |
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Target: 5'- uUCACCGUCACUgACCGGcguGCCGCC-CUg -3' miRNA: 3'- -GGUGGUGGUGA-UGGUCu--CGGCGGcGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 6495 | 0.67 | 0.329982 |
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Target: 5'- gCGCCACCGC--CCAGcGCauaucCCGCCa -3' miRNA: 3'- gGUGGUGGUGauGGUCuCGgc---GGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 13690 | 0.67 | 0.338111 |
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Target: 5'- --uUCGggUCAUUGcCCAGcGCCGCCGCCa -3' miRNA: 3'- gguGGU--GGUGAU-GGUCuCGGCGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 21665 | 0.66 | 0.372065 |
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Target: 5'- aCCACgGCCACaACCGGccAGCUaauaGCC-CCg -3' miRNA: 3'- -GGUGgUGGUGaUGGUC--UCGG----CGGcGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 1306 | 0.66 | 0.389892 |
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Target: 5'- cUCACCGCCGgUAUCAGcaCC-CCGCUg -3' miRNA: 3'- -GGUGGUGGUgAUGGUCucGGcGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 10489 | 0.66 | 0.408268 |
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Target: 5'- uCCugCgcgaugggucaGCCACUgaaGCCAGucgguGCacauGCCGCCg -3' miRNA: 3'- -GGugG-----------UGGUGA---UGGUCu----CGg---CGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 1392 | 0.66 | 0.408268 |
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Target: 5'- gCGCCACCuAUgagGCCAGccgcagcgaggaAGCCaGCCacGCCg -3' miRNA: 3'- gGUGGUGG-UGa--UGGUC------------UCGG-CGG--CGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 7826 | 1.12 | 0.000149 |
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Target: 5'- gCCACCACCACUACCAGAGCCGCCGCCc -3' miRNA: 3'- -GGUGGUGGUGAUGGUCUCGGCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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