Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7115 | 5' | -45.1 | NC_001895.1 | + | 9409 | 0.66 | 0.989139 |
Target: 5'- gGUCGCUCAAUGUUUAGagGUuUAUgAg -3' miRNA: 3'- -CGGCGAGUUACAAAUUaaCGcGUAgU- -5' |
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7115 | 5' | -45.1 | NC_001895.1 | + | 20402 | 0.66 | 0.985416 |
Target: 5'- cGCUGC-CGGUGUgggGAUUGCGgCggCGg -3' miRNA: 3'- -CGGCGaGUUACAaa-UUAACGC-GuaGU- -5' |
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7115 | 5' | -45.1 | NC_001895.1 | + | 21915 | 0.66 | 0.980789 |
Target: 5'- uGCCGCUUAAUGcgUUcaacaAAcUGCGCAg-- -3' miRNA: 3'- -CGGCGAGUUACa-AA-----UUaACGCGUagu -5' |
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7115 | 5' | -45.1 | NC_001895.1 | + | 1932 | 0.68 | 0.955925 |
Target: 5'- aGCUGCUCAAUGUcgAAcaGCGUG-CAg -3' miRNA: 3'- -CGGCGAGUUACAaaUUaaCGCGUaGU- -5' |
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7115 | 5' | -45.1 | NC_001895.1 | + | 10882 | 0.71 | 0.875515 |
Target: 5'- cGCCgguguGUUCAAUGUUgcc--GCGCAUCGu -3' miRNA: 3'- -CGG-----CGAGUUACAAauuaaCGCGUAGU- -5' |
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7115 | 5' | -45.1 | NC_001895.1 | + | 9436 | 1.13 | 0.003335 |
Target: 5'- gGCCGCUCAAUGUUUAAUUGCGCAUCAg -3' miRNA: 3'- -CGGCGAGUUACAAAUUAACGCGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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