Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7116 | 3' | -51.3 | NC_001895.1 | + | 27177 | 0.66 | 0.792275 |
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Target: 5'- cACGCGGGauaacuccgccuuugUGAUGUggcgaAUCAUGGCa -3' miRNA: 3'- -UGCGCCCa--------------ACUACGaaag-UGGUACUG- -5' |
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| 7116 | 3' | -51.3 | NC_001895.1 | + | 13638 | 0.67 | 0.725079 |
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Target: 5'- gGCGCuGGGcaaUGAcccgaauuuUGCUacCACCAUGACu -3' miRNA: 3'- -UGCG-CCCa--ACU---------ACGAaaGUGGUACUG- -5' |
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| 7116 | 3' | -51.3 | NC_001895.1 | + | 25695 | 0.68 | 0.678647 |
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Target: 5'- uUGUGGGgucugcagGAUGU--UCAUCAUGACa -3' miRNA: 3'- uGCGCCCaa------CUACGaaAGUGGUACUG- -5' |
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| 7116 | 3' | -51.3 | NC_001895.1 | + | 6841 | 0.69 | 0.572415 |
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Target: 5'- gACGCGGGgUGAUaggUUCACCAccGGCa -3' miRNA: 3'- -UGCGCCCaACUAcgaAAGUGGUa-CUG- -5' |
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| 7116 | 3' | -51.3 | NC_001895.1 | + | 6359 | 0.71 | 0.492442 |
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Target: 5'- gGCGCGcucGUaguaaaaaaccuUGAUGCUUUCACCGUcGAUa -3' miRNA: 3'- -UGCGCc--CA------------ACUACGAAAGUGGUA-CUG- -5' |
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| 7116 | 3' | -51.3 | NC_001895.1 | + | 10779 | 1.09 | 0.001191 |
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Target: 5'- gACGCGGGUUGAUGCUUUCACCAUGACc -3' miRNA: 3'- -UGCGCCCAACUACGAAAGUGGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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