Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7117 | 5' | -58.5 | NC_001895.1 | + | 11757 | 0.66 | 0.376243 |
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Target: 5'- uGUCACCaGUCCGUcucCGGCCUgugUCACc -3' miRNA: 3'- -UAGUGG-CGGGCAc--GCCGGAguaAGUG- -5' |
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| 7117 | 5' | -58.5 | NC_001895.1 | + | 1737 | 0.66 | 0.376243 |
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Target: 5'- -gCACCGCCgGUgGUGGCCgguggCAaaUUCAg -3' miRNA: 3'- uaGUGGCGGgCA-CGCCGGa----GU--AAGUg -5' |
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| 7117 | 5' | -58.5 | NC_001895.1 | + | 18594 | 0.68 | 0.285714 |
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Target: 5'- -cCACCGCCCacauGUGCG-CCUCAgcCAUc -3' miRNA: 3'- uaGUGGCGGG----CACGCcGGAGUaaGUG- -5' |
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| 7117 | 5' | -58.5 | NC_001895.1 | + | 18253 | 0.68 | 0.264206 |
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Target: 5'- -cCACCGCCCGUGCgcuggGGCUg----CGCg -3' miRNA: 3'- uaGUGGCGGGCACG-----CCGGaguaaGUG- -5' |
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| 7117 | 5' | -58.5 | NC_001895.1 | + | 165 | 0.69 | 0.250609 |
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Target: 5'- --aGCCGCCCGcagGCGGCgaUGUUCAg -3' miRNA: 3'- uagUGGCGGGCa--CGCCGgaGUAAGUg -5' |
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| 7117 | 5' | -58.5 | NC_001895.1 | + | 13104 | 0.7 | 0.207525 |
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Target: 5'- cAUCAUCGUCagcaGUGUGGCCUCAguggaGCu -3' miRNA: 3'- -UAGUGGCGGg---CACGCCGGAGUaag--UG- -5' |
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| 7117 | 5' | -58.5 | NC_001895.1 | + | 218 | 0.73 | 0.128779 |
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Target: 5'- cAUCGCCGCCUGcggGCGGCUUU-UUUACa -3' miRNA: 3'- -UAGUGGCGGGCa--CGCCGGAGuAAGUG- -5' |
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| 7117 | 5' | -58.5 | NC_001895.1 | + | 11553 | 1.08 | 0.000251 |
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Target: 5'- cAUCACCGCCCGUGCGGCCUCAUUCACa -3' miRNA: 3'- -UAGUGGCGGGCACGCCGGAGUAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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