Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7118 | 5' | -53.4 | NC_001895.1 | + | 2572 | 0.66 | 0.674682 |
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Target: 5'- cUGGUAucGUGCCGGgCUUgagcaCCGCa-UCCg -3' miRNA: 3'- cACCAU--UACGGCUgGAA-----GGCGgaAGG- -5' |
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| 7118 | 5' | -53.4 | NC_001895.1 | + | 8092 | 0.67 | 0.623936 |
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Target: 5'- aUGGUGAuggucgucUGCUGACCgcuggcgacguugUUCGCCcugUCCa -3' miRNA: 3'- cACCAUU--------ACGGCUGGa------------AGGCGGa--AGG- -5' |
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| 7118 | 5' | -53.4 | NC_001895.1 | + | 6457 | 0.67 | 0.605469 |
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Target: 5'- aUGGUGcugucaAUGCUGucaGCCUUCuuguCGCCUUUCg -3' miRNA: 3'- cACCAU------UACGGC---UGGAAG----GCGGAAGG- -5' |
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| 7118 | 5' | -53.4 | NC_001895.1 | + | 5689 | 0.68 | 0.537124 |
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Target: 5'- gGUGGUGAUGCCG-CCggUgCGCUggCg -3' miRNA: 3'- -CACCAUUACGGCuGGa-AgGCGGaaGg -5' |
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| 7118 | 5' | -53.4 | NC_001895.1 | + | 22773 | 0.69 | 0.511594 |
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Target: 5'- cUGGUcgccauacUGCCGGCCUuaccgaugagcgauUCCGCCUguuuaCCg -3' miRNA: 3'- cACCAuu------ACGGCUGGA--------------AGGCGGAa----GG- -5' |
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| 7118 | 5' | -53.4 | NC_001895.1 | + | 825 | 0.7 | 0.428292 |
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Target: 5'- cUGGUGGgUGCCGuCCUUCaacgagccgacaGCCUUCg -3' miRNA: 3'- cACCAUU-ACGGCuGGAAGg-----------CGGAAGg -5' |
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| 7118 | 5' | -53.4 | NC_001895.1 | + | 11513 | 0.8 | 0.10718 |
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Target: 5'- -cGGUGAUGCUGGCCUgugCCGCCg--- -3' miRNA: 3'- caCCAUUACGGCUGGAa--GGCGGaagg -5' |
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| 7118 | 5' | -53.4 | NC_001895.1 | + | 12849 | 1.11 | 0.000544 |
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Target: 5'- cGUGGUAAUGCCGACCUUCCGCCUUCCc -3' miRNA: 3'- -CACCAUUACGGCUGGAAGGCGGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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