Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7120 | 3' | -54.1 | NC_001895.1 | + | 2524 | 0.66 | 0.63033 |
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Target: 5'- -cCGCCAGaUUGGCGcgACGUUuuauuuUUCCCg -3' miRNA: 3'- caGCGGUCcGACCGU--UGUAGu-----AAGGG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 2984 | 0.66 | 0.617576 |
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Target: 5'- cGUCGUCAGGCggcgcugcuguacUGGCAGgGgu-UUUCCg -3' miRNA: 3'- -CAGCGGUCCG-------------ACCGUUgUaguAAGGG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 4744 | 0.66 | 0.595596 |
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Target: 5'- -gUGCaucaAGGCUGGCAuaAUCAccgCCCu -3' miRNA: 3'- caGCGg---UCCGACCGUugUAGUaa-GGG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 8038 | 0.68 | 0.494241 |
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Target: 5'- -gCGCgCAGGCUGGCAuuuuUGUguUUCaCCa -3' miRNA: 3'- caGCG-GUCCGACCGUu---GUAguAAG-GG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 12316 | 0.69 | 0.4517 |
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Target: 5'- -cUGCCGuGGCUGGCGugGgCGUUUUCg -3' miRNA: 3'- caGCGGU-CCGACCGUugUaGUAAGGG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 5748 | 0.69 | 0.4517 |
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Target: 5'- -gCGCCAGcGCaccGGCGGCAUCAccaCCg -3' miRNA: 3'- caGCGGUC-CGa--CCGUUGUAGUaagGG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 17811 | 0.69 | 0.441379 |
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Target: 5'- --gGCCGGuaaaaaaggcacGCUGGCGGCAUCGUUgCa -3' miRNA: 3'- cagCGGUC------------CGACCGUUGUAGUAAgGg -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 3946 | 0.7 | 0.363954 |
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Target: 5'- -aUGCCuGGCUGGUuuCAUCcg-CCCu -3' miRNA: 3'- caGCGGuCCGACCGuuGUAGuaaGGG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 8084 | 0.71 | 0.328926 |
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Target: 5'- gGUCGUCugcugaccGCUGGCGACGUUGUUCgCCc -3' miRNA: 3'- -CAGCGGuc------CGACCGUUGUAGUAAG-GG- -5' |
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| 7120 | 3' | -54.1 | NC_001895.1 | + | 13826 | 1.11 | 0.000454 |
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Target: 5'- gGUCGCCAGGCUGGCAACAUCAUUCCCc -3' miRNA: 3'- -CAGCGGUCCGACCGUUGUAGUAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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