miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7121 3' -54 NC_001895.1 + 3073 0.66 0.638011
Target:  5'- aGCCAU-CCGGgacUGAUGGCgGaGGAUGCg -3'
miRNA:   3'- -UGGUAcGGCU---GUUACCGgCaCCUAUG- -5'
7121 3' -54 NC_001895.1 + 22931 0.66 0.591634
Target:  5'- aACgAUGCCGACg--GGCagGUcGGGUGCc -3'
miRNA:   3'- -UGgUACGGCUGuuaCCGg-CA-CCUAUG- -5'
7121 3' -54 NC_001895.1 + 7279 0.66 0.590479
Target:  5'- gGCCAgaacagguaaagcUGCUGAUAGcgaccggaGGCCGUGGAUu- -3'
miRNA:   3'- -UGGU-------------ACGGCUGUUa-------CCGGCACCUAug -5'
7121 3' -54 NC_001895.1 + 21502 0.68 0.512279
Target:  5'- cGCCAgugcaccGCCGGCAAUGauGCCGaUGGuugACg -3'
miRNA:   3'- -UGGUa------CGGCUGUUAC--CGGC-ACCua-UG- -5'
7121 3' -54 NC_001895.1 + 21595 0.7 0.411559
Target:  5'- cGCCAUcggggcuauuagcugGCCGGuuGUGGCCGUGGugGCu -3'
miRNA:   3'- -UGGUA---------------CGGCUguUACCGGCACCuaUG- -5'
7121 3' -54 NC_001895.1 + 10495 0.74 0.205804
Target:  5'- --gGUGCUGAUucUGGCCGUGGGUGg -3'
miRNA:   3'- uggUACGGCUGuuACCGGCACCUAUg -5'
7121 3' -54 NC_001895.1 + 17759 1.1 0.000504
Target:  5'- gACCAUGCCGACAAUGGCCGUGGAUACg -3'
miRNA:   3'- -UGGUACGGCUGUUACCGGCACCUAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.