Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7122 | 5' | -54.9 | NC_001895.1 | + | 6116 | 0.66 | 0.604903 |
Target: 5'- --cCCGAagcgcGUCCGGGAAcguaugcGCC-UUGAGCa -3' miRNA: 3'- auuGGCU-----UAGGCCCUU-------CGGuGGCUCG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 5156 | 0.66 | 0.594631 |
Target: 5'- uUAGCCG-GUgUGGaGAAGUCACCGAc- -3' miRNA: 3'- -AUUGGCuUAgGCC-CUUCGGUGGCUcg -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 2130 | 0.67 | 0.549412 |
Target: 5'- gAACUGuuuaaCCGGGGAcgcgccagcGCCGCCGAacGCg -3' miRNA: 3'- aUUGGCuua--GGCCCUU---------CGGUGGCU--CG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 9180 | 0.67 | 0.538262 |
Target: 5'- cAAUgGAAUUuuacggCGGGAAGUCGCCGAa- -3' miRNA: 3'- aUUGgCUUAG------GCCCUUCGGUGGCUcg -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 1077 | 0.68 | 0.462806 |
Target: 5'- -uACCG-GUCUGGcaaaAAGCCugCGGGCu -3' miRNA: 3'- auUGGCuUAGGCCc---UUCGGugGCUCG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 6592 | 0.68 | 0.452473 |
Target: 5'- -cGCCGcaAAUCCGGGAcuGGCUgaACUGGGg -3' miRNA: 3'- auUGGC--UUAGGCCCU--UCGG--UGGCUCg -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 19591 | 0.69 | 0.432191 |
Target: 5'- cUGACCGAG-Ca-GGAAGUCGCCGcGCu -3' miRNA: 3'- -AUUGGCUUaGgcCCUUCGGUGGCuCG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 22509 | 0.69 | 0.393277 |
Target: 5'- -cGCCGGucaGUUCGGGAuaaAGCaCACCGgccAGCg -3' miRNA: 3'- auUGGCU---UAGGCCCU---UCG-GUGGC---UCG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 5197 | 0.71 | 0.290979 |
Target: 5'- aUGACCGcgcagCGGGAAGCCGCCccGCu -3' miRNA: 3'- -AUUGGCuuag-GCCCUUCGGUGGcuCG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 13875 | 0.72 | 0.276103 |
Target: 5'- aAACCGAG-CCGGGAa--CGCCGuGCa -3' miRNA: 3'- aUUGGCUUaGGCCCUucgGUGGCuCG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 21228 | 0.76 | 0.14603 |
Target: 5'- aAGCCucagaCGGGAAGaCCGCCGAGCu -3' miRNA: 3'- aUUGGcuuagGCCCUUC-GGUGGCUCG- -5' |
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7122 | 5' | -54.9 | NC_001895.1 | + | 19083 | 1.11 | 0.000399 |
Target: 5'- aUAACCGAAUCCGGGAAGCCACCGAGCg -3' miRNA: 3'- -AUUGGCUUAGGCCCUUCGGUGGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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