Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7126 | 3' | -42.6 | NC_001895.1 | + | 24372 | 0.66 | 0.997574 |
Target: 5'- gUAGGGGCUUUCAacGGUAc------ -3' miRNA: 3'- aAUUCCCGAAAGUcaCCAUuaaauac -5' |
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7126 | 3' | -42.6 | NC_001895.1 | + | 24255 | 0.67 | 0.996361 |
Target: 5'- -gAGGGGCUUUUuugucgauGUGGUcAAUgUGUGg -3' miRNA: 3'- aaUUCCCGAAAGu-------CACCA-UUAaAUAC- -5' |
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7126 | 3' | -42.6 | NC_001895.1 | + | 111 | 0.67 | 0.993645 |
Target: 5'- --cGGGGUguuuugugCGGUGGUGAgcgUGUGa -3' miRNA: 3'- aauUCCCGaaa-----GUCACCAUUaa-AUAC- -5' |
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7126 | 3' | -42.6 | NC_001895.1 | + | 11674 | 0.68 | 0.987536 |
Target: 5'- uUUGAGGGCUUaagcgucgccgggUCAGUGGg-------- -3' miRNA: 3'- -AAUUCCCGAA-------------AGUCACCauuaaauac -5' |
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7126 | 3' | -42.6 | NC_001895.1 | + | 24468 | 1.07 | 0.014383 |
Target: 5'- uUUAAGGGCUUUCAGUGGUAAUUUAUGc -3' miRNA: 3'- -AAUUCCCGAAAGUCACCAUUAAAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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