Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7130 | 5' | -48.4 | NC_001895.1 | + | 28795 | 0.66 | 0.914381 |
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Target: 5'- uGUUUACUgGCauaaGGAGAACGGUCUUu -3' miRNA: 3'- gUAAGUGGgCGga--CUUUUUGUCAGAG- -5' |
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| 7130 | 5' | -48.4 | NC_001895.1 | + | 8855 | 0.66 | 0.899894 |
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Target: 5'- --gUCACCCGCCcGAuGGAGCuGUauaUCa -3' miRNA: 3'- guaAGUGGGCGGaCU-UUUUGuCAg--AG- -5' |
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| 7130 | 5' | -48.4 | NC_001895.1 | + | 5458 | 0.66 | 0.892163 |
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Target: 5'- --aUUGCCCGuuaugcCCUGAAGuACGGUCUg -3' miRNA: 3'- guaAGUGGGC------GGACUUUuUGUCAGAg -5' |
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| 7130 | 5' | -48.4 | NC_001895.1 | + | 29374 | 0.67 | 0.87059 |
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Target: 5'- ---cCGCCCugguuaagguguuuuGCCUGAAA-ACGGUUUCg -3' miRNA: 3'- guaaGUGGG---------------CGGACUUUuUGUCAGAG- -5' |
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| 7130 | 5' | -48.4 | NC_001895.1 | + | 5788 | 0.67 | 0.867089 |
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Target: 5'- ---aCGCCUGCCUGA----CAGUCg- -3' miRNA: 3'- guaaGUGGGCGGACUuuuuGUCAGag -5' |
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| 7130 | 5' | -48.4 | NC_001895.1 | + | 3911 | 1.11 | 0.001782 |
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Target: 5'- gCAUUCACCCGCCUGAAAAACAGUCUCg -3' miRNA: 3'- -GUAAGUGGGCGGACUUUUUGUCAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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