Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7132 | 3' | -55.8 | NC_001895.1 | + | 7636 | 0.66 | 0.538012 |
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Target: 5'- gGUUGCCGGUGG---UGUGGGcguguaUGCCa -3' miRNA: 3'- gCGACGGCCACUcagACACCU------AUGGg -5' |
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| 7132 | 3' | -55.8 | NC_001895.1 | + | 20618 | 0.66 | 0.538012 |
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Target: 5'- uGCgaaaGCCGGUGGGgaUGUuGAUGCCa -3' miRNA: 3'- gCGa---CGGCCACUCagACAcCUAUGGg -5' |
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| 7132 | 3' | -55.8 | NC_001895.1 | + | 1590 | 0.67 | 0.494796 |
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Target: 5'- aCGUUGUauuuuuCGGUGAGUUUGcGGAUGgauUCCg -3' miRNA: 3'- -GCGACG------GCCACUCAGACaCCUAU---GGG- -5' |
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| 7132 | 3' | -55.8 | NC_001895.1 | + | 20401 | 0.69 | 0.394653 |
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Target: 5'- gCGCUGCCGGUGuGg----GGAUugCg -3' miRNA: 3'- -GCGACGGCCACuCagacaCCUAugGg -5' |
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| 7132 | 3' | -55.8 | NC_001895.1 | + | 2069 | 0.69 | 0.358423 |
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Target: 5'- aCGCccuUGCCGGUGGGcuucUCUGUgcGGAcgGCCa -3' miRNA: 3'- -GCG---ACGGCCACUC----AGACA--CCUa-UGGg -5' |
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| 7132 | 3' | -55.8 | NC_001895.1 | + | 1365 | 0.71 | 0.293185 |
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Target: 5'- cCGCUGCUGGUcauGGUCuUGcGGAUAgCCa -3' miRNA: 3'- -GCGACGGCCAc--UCAG-ACaCCUAUgGG- -5' |
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| 7132 | 3' | -55.8 | NC_001895.1 | + | 5564 | 1.13 | 0.000244 |
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Target: 5'- aCGCUGCCGGUGAGUCUGUGGAUACCCg -3' miRNA: 3'- -GCGACGGCCACUCAGACACCUAUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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