Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7132 | 5' | -56 | NC_001895.1 | + | 6128 | 0.66 | 0.529727 |
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Target: 5'- cCGGGaacguauGCgCCUUGAGCaGACCGUCGCc -3' miRNA: 3'- uGCCUc------UG-GGAACUUGaCUGGCGGUG- -5' |
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| 7132 | 5' | -56 | NC_001895.1 | + | 19721 | 0.67 | 0.46551 |
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Target: 5'- gGCGGAuguGGCag-UGAuauuucACUGGCCGCCAUc -3' miRNA: 3'- -UGCCU---CUGggaACU------UGACUGGCGGUG- -5' |
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| 7132 | 5' | -56 | NC_001895.1 | + | 8220 | 0.68 | 0.425016 |
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Target: 5'- uACGGugcuGACCUgccUGAuGCUGugCGCCGg -3' miRNA: 3'- -UGCCu---CUGGGa--ACU-UGACugGCGGUg -5' |
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| 7132 | 5' | -56 | NC_001895.1 | + | 3781 | 0.68 | 0.409417 |
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Target: 5'- aGCGGuGACCgCUguugcUGAacaugugcaggaaaaACUGAgCGCCACu -3' miRNA: 3'- -UGCCuCUGG-GA-----ACU---------------UGACUgGCGGUG- -5' |
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| 7132 | 5' | -56 | NC_001895.1 | + | 23418 | 0.7 | 0.317202 |
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Target: 5'- uAUGGuGACCUgcggGAuuuuuuuaccGCUGGCCGUCACu -3' miRNA: 3'- -UGCCuCUGGGaa--CU----------UGACUGGCGGUG- -5' |
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| 7132 | 5' | -56 | NC_001895.1 | + | 5598 | 1.1 | 0.000358 |
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Target: 5'- gACGGAGACCCUUGAACUGACCGCCACg -3' miRNA: 3'- -UGCCUCUGGGAACUUGACUGGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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