Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7136 | 5' | -56.8 | NC_001895.1 | + | 18282 | 0.66 | 0.457048 |
Target: 5'- ----aCGCGGugGCGGuGGUACUGGu- -3' miRNA: 3'- acuagGCGCUugCGCC-UCGUGACCua -5' |
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7136 | 5' | -56.8 | NC_001895.1 | + | 8313 | 0.66 | 0.446842 |
Target: 5'- -cGUCgGUGcAgGCGGGGCGCUGGu- -3' miRNA: 3'- acUAGgCGCuUgCGCCUCGUGACCua -5' |
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7136 | 5' | -56.8 | NC_001895.1 | + | 5883 | 0.69 | 0.326923 |
Target: 5'- cGcUCCGgGucuuACGCGGGGCGC-GGGUg -3' miRNA: 3'- aCuAGGCgCu---UGCGCCUCGUGaCCUA- -5' |
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7136 | 5' | -56.8 | NC_001895.1 | + | 7314 | 1.06 | 0.000524 |
Target: 5'- uUGAUCCGCGAACGCGGAGCACUGGAUg -3' miRNA: 3'- -ACUAGGCGCUUGCGCCUCGUGACCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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