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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7137 | 3' | -62.5 | NC_001895.1 | + | 21403 | 0.66 | 0.208046 |
Target: 5'- cGUgAGCUUacgcaGCGCGCCUuccUGCUGGuCAa -3' miRNA: 3'- aCGgUCGGA-----CGCGCGGA---GCGACCuGU- -5' |
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7137 | 3' | -62.5 | NC_001895.1 | + | 21168 | 0.67 | 0.202563 |
Target: 5'- cUGCCGuGCugaugaCUGC-CGCCUCaaccggaaaGCUGGACAa -3' miRNA: 3'- -ACGGU-CG------GACGcGCGGAG---------CGACCUGU- -5' |
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7137 | 3' | -62.5 | NC_001895.1 | + | 3823 | 0.69 | 0.142043 |
Target: 5'- cGCCAcUgaGCaGCGCCUCGCUGaGAUg -3' miRNA: 3'- aCGGUcGgaCG-CGCGGAGCGAC-CUGu -5' |
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7137 | 3' | -62.5 | NC_001895.1 | + | 1361 | 0.73 | 0.067775 |
Target: 5'- aGCCAGCCacGC-CGaCCUCGCcUGGGCGa -3' miRNA: 3'- aCGGUCGGa-CGcGC-GGAGCG-ACCUGU- -5' |
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7137 | 3' | -62.5 | NC_001895.1 | + | 7990 | 1.08 | 0.000095 |
Target: 5'- aUGCCAGCCUGCGCGCCUCGCUGGACAg -3' miRNA: 3'- -ACGGUCGGACGCGCGGAGCGACCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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