Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7138 | 3' | -57.2 | NC_001895.1 | + | 4359 | 0.66 | 0.405573 |
Target: 5'- ---cCGGCAGUGUCGGuaGUGcUGGCGa -3' miRNA: 3'- cucaGUCGUCGCAGCC--CACuACCGUg -5' |
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7138 | 3' | -57.2 | NC_001895.1 | + | 22943 | 0.68 | 0.349861 |
Target: 5'- ----gGGCAG-GUCGGGUGccgguucGUGGCGCu -3' miRNA: 3'- cucagUCGUCgCAGCCCAC-------UACCGUG- -5' |
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7138 | 3' | -57.2 | NC_001895.1 | + | 12801 | 0.71 | 0.207372 |
Target: 5'- aGGUCGGCauuaccacgauGGcCGUCGGG-GAUGGCGg -3' miRNA: 3'- cUCAGUCG-----------UC-GCAGCCCaCUACCGUg -5' |
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7138 | 3' | -57.2 | NC_001895.1 | + | 9012 | 1.1 | 0.000239 |
Target: 5'- aGAGUCAGCAGCGUCGGGUGAUGGCACa -3' miRNA: 3'- -CUCAGUCGUCGCAGCCCACUACCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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