Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7138 | 5' | -59.5 | NC_001895.1 | + | 7347 | 0.7 | 0.202742 |
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Target: 5'- cGGGCGGgACGCauugaGCGCGCCauuucACGCUg -3' miRNA: 3'- -CCUGCCgUGUGgga--UGCGCGG-----UGCGG- -5' |
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| 7138 | 5' | -59.5 | NC_001895.1 | + | 7406 | 0.71 | 0.192214 |
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Target: 5'- -aAUGGCGCGCUCaAUGCGUCcCGCCc -3' miRNA: 3'- ccUGCCGUGUGGGaUGCGCGGuGCGG- -5' |
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| 7138 | 5' | -59.5 | NC_001895.1 | + | 22610 | 0.72 | 0.153509 |
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Target: 5'- aGACGGCACACCCcggaaaauugauuuUACGCuGUCACucacCCg -3' miRNA: 3'- cCUGCCGUGUGGG--------------AUGCG-CGGUGc---GG- -5' |
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| 7138 | 5' | -59.5 | NC_001895.1 | + | 6454 | 0.74 | 0.117322 |
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Target: 5'- cGGugGcGCGCAUCCaggGCaaGCCGCGCUg -3' miRNA: 3'- -CCugC-CGUGUGGGa--UGcgCGGUGCGG- -5' |
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| 7138 | 5' | -59.5 | NC_001895.1 | + | 6514 | 0.79 | 0.045575 |
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Target: 5'- -cGCGGCuuGCCCUggauGCGCGCCAcCGCCc -3' miRNA: 3'- ccUGCCGugUGGGA----UGCGCGGU-GCGG- -5' |
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| 7138 | 5' | -59.5 | NC_001895.1 | + | 9048 | 1.14 | 0.000097 |
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Target: 5'- cGGACGGCACACCCUACGCGCCACGCCa -3' miRNA: 3'- -CCUGCCGUGUGGGAUGCGCGGUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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