Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7143 | 3' | -44.6 | NC_001895.1 | + | 16804 | 0.66 | 0.991078 |
Target: 5'- ---gUGAAAAUGCCuuaagauaauACUCAUCUAUUGc -3' miRNA: 3'- guugACUUUUAUGG----------UGAGUGGGUAAU- -5' |
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7143 | 3' | -44.6 | NC_001895.1 | + | 33475 | 0.68 | 0.961046 |
Target: 5'- uGACUGGAAAUACUgaUgGCUCAUUAg -3' miRNA: 3'- gUUGACUUUUAUGGugAgUGGGUAAU- -5' |
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7143 | 3' | -44.6 | NC_001895.1 | + | 15679 | 0.68 | 0.961046 |
Target: 5'- gCGAgUGGAAAacacuUGCCAUUCACCuCAUa- -3' miRNA: 3'- -GUUgACUUUU-----AUGGUGAGUGG-GUAau -5' |
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7143 | 3' | -44.6 | NC_001895.1 | + | 17591 | 1.08 | 0.007305 |
Target: 5'- gCAACUGAAAAUACCACUCACCCAUUAa -3' miRNA: 3'- -GUUGACUUUUAUGGUGAGUGGGUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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