Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7144 | 5' | -54.1 | NC_001895.1 | + | 20381 | 0.66 | 0.680238 |
Target: 5'- cGGAaauauggccucAGugGGCGCUGccGGUGugGGGa -3' miRNA: 3'- -CCU-----------UCugCCGUGGCcaCUACugCUCc -5' |
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7144 | 5' | -54.1 | NC_001895.1 | + | 5916 | 0.66 | 0.66903 |
Target: 5'- -cAGGGCGGCacGCCGGUcaGUGACGGu- -3' miRNA: 3'- ccUUCUGCCG--UGGCCAc-UACUGCUcc -5' |
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7144 | 5' | -54.1 | NC_001895.1 | + | 12797 | 0.69 | 0.506625 |
Target: 5'- cGGAAGGuCGGCAuuaccacgauggccgUCGGgGAUGGCGGuGGu -3' miRNA: 3'- -CCUUCU-GCCGU---------------GGCCaCUACUGCU-CC- -5' |
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7144 | 5' | -54.1 | NC_001895.1 | + | 7766 | 0.69 | 0.502356 |
Target: 5'- cGGgcGGCGGCucugguAgUGGUGGUGGCGAc- -3' miRNA: 3'- -CCuuCUGCCG------UgGCCACUACUGCUcc -5' |
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7144 | 5' | -54.1 | NC_001895.1 | + | 7617 | 0.69 | 0.490695 |
Target: 5'- uGgcGACGGUuaucugugugguuGCCGGUGGUGugGGc- -3' miRNA: 3'- cCuuCUGCCG-------------UGGCCACUACugCUcc -5' |
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7144 | 5' | -54.1 | NC_001895.1 | + | 11826 | 0.73 | 0.313847 |
Target: 5'- cGAAGACcugcuGGCugUGGUGcguaacgcccuuaAUGGCGAGGa -3' miRNA: 3'- cCUUCUG-----CCGugGCCAC-------------UACUGCUCC- -5' |
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7144 | 5' | -54.1 | NC_001895.1 | + | 17891 | 1.13 | 0.000456 |
Target: 5'- uGGAAGACGGCACCGGUGAUGACGAGGa -3' miRNA: 3'- -CCUUCUGCCGUGGCCACUACUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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