Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7147 | 3' | -55.4 | NC_001895.1 | + | 1765 | 0.66 | 0.514274 |
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Target: 5'- -gGCGgGAGCcGAACGGaUugGCGg- -3' miRNA: 3'- gaCGCgCUCGaCUUGCCaGugCGUag -5' |
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| 7147 | 3' | -55.4 | NC_001895.1 | + | 8506 | 0.67 | 0.471377 |
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Target: 5'- cCUGCGCaGGCUGucgGGUUugaGCAUCg -3' miRNA: 3'- -GACGCGcUCGACuugCCAGug-CGUAG- -5' |
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| 7147 | 3' | -55.4 | NC_001895.1 | + | 18178 | 0.67 | 0.460935 |
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Target: 5'- -aGCGUGAGCUGAugGucaUCugGCc-- -3' miRNA: 3'- gaCGCGCUCGACUugCc--AGugCGuag -5' |
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| 7147 | 3' | -55.4 | NC_001895.1 | + | 18954 | 0.69 | 0.372987 |
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Target: 5'- -gGUGCGGGgUGAACGGcaGCGCAc- -3' miRNA: 3'- gaCGCGCUCgACUUGCCagUGCGUag -5' |
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| 7147 | 3' | -55.4 | NC_001895.1 | + | 6436 | 0.74 | 0.177208 |
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Target: 5'- -gGCGgGAuaugcGCUGGGCGGUggCGCGCAUCc -3' miRNA: 3'- gaCGCgCU-----CGACUUGCCA--GUGCGUAG- -5' |
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| 7147 | 3' | -55.4 | NC_001895.1 | + | 19953 | 1 | 0.002191 |
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Target: 5'- aCUGCGCG-GCUGAACGGUCACGCAUCc -3' miRNA: 3'- -GACGCGCuCGACUUGCCAGUGCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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