Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7147 | 5' | -52.8 | NC_001895.1 | + | 25817 | 0.66 | 0.731486 |
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Target: 5'- aGGGGUUCCGUAuGGCac--GCugAGUu -3' miRNA: 3'- -CCCUAAGGCGUuCUGgucaCGugUCG- -5' |
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| 7147 | 5' | -52.8 | NC_001895.1 | + | 8301 | 0.66 | 0.709006 |
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Target: 5'- cGGGGcgCUGguGcaACCGGcGCACAGCa -3' miRNA: 3'- -CCCUaaGGCguUc-UGGUCaCGUGUCG- -5' |
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| 7147 | 5' | -52.8 | NC_001895.1 | + | 21518 | 0.66 | 0.697634 |
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Target: 5'- -cGAUgaugCCaGUAAGcGCCAGUGCACcGCc -3' miRNA: 3'- ccCUAa---GG-CGUUC-UGGUCACGUGuCG- -5' |
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| 7147 | 5' | -52.8 | NC_001895.1 | + | 5138 | 0.66 | 0.686191 |
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Target: 5'- cGGGAgcguaaCCGau-GACgAGUcccGCACAGCg -3' miRNA: 3'- -CCCUaa----GGCguuCUGgUCA---CGUGUCG- -5' |
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| 7147 | 5' | -52.8 | NC_001895.1 | + | 1428 | 0.71 | 0.399695 |
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Target: 5'- uGGcuaUCCGCAAGACCA-UGaccaGCAGCg -3' miRNA: 3'- cCCua-AGGCGUUCUGGUcACg---UGUCG- -5' |
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| 7147 | 5' | -52.8 | NC_001895.1 | + | 19987 | 1.14 | 0.000384 |
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Target: 5'- aGGGAUUCCGCAAGACCAGUGCACAGCu -3' miRNA: 3'- -CCCUAAGGCGUUCUGGUCACGUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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