Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7148 | 3' | -53.6 | NC_001895.1 | + | 18044 | 0.66 | 0.631757 |
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Target: 5'- ---cGACUCCGCCGccagcAUGGCUuuuaguccgguGUACu -3' miRNA: 3'- uuuuCUGAGGCGGCuu---UACCGG-----------CGUG- -5' |
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| 7148 | 3' | -53.6 | NC_001895.1 | + | 24166 | 0.66 | 0.584915 |
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Target: 5'- cGGGAGAaguccaCGCCGuaa-GGCCGCACc -3' miRNA: 3'- -UUUUCUgag---GCGGCuuuaCCGGCGUG- -5' |
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| 7148 | 3' | -53.6 | NC_001895.1 | + | 20882 | 0.67 | 0.527432 |
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Target: 5'- uGAAGAaaCCGCCGcAAUGGUCgggGCGCu -3' miRNA: 3'- uUUUCUgaGGCGGCuUUACCGG---CGUG- -5' |
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| 7148 | 3' | -53.6 | NC_001895.1 | + | 2011 | 0.68 | 0.493954 |
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Target: 5'- uGAGGACgCC-CUGAAAggcGGCUGCACg -3' miRNA: 3'- uUUUCUGaGGcGGCUUUa--CCGGCGUG- -5' |
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| 7148 | 3' | -53.6 | NC_001895.1 | + | 7691 | 0.7 | 0.38088 |
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Target: 5'- cGGGAG-CUgCCGCCGGAAucgcUGGCCGUg- -3' miRNA: 3'- -UUUUCuGA-GGCGGCUUU----ACCGGCGug -5' |
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| 7148 | 3' | -53.6 | NC_001895.1 | + | 20515 | 1.07 | 0.000836 |
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Target: 5'- cAAAAGACUCCGCCGAAAUGGCCGCACu -3' miRNA: 3'- -UUUUCUGAGGCGGCUUUACCGGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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