Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7148 | 5' | -57.6 | NC_001895.1 | + | 20398 | 0.75 | 0.111368 |
Target: 5'- uGGGCGCuGCCGGUguggggauugCGGCGGCGGgaacgaugGCCg -3' miRNA: 3'- -UCCGCG-CGGUCGa---------GCCGCUGUUa-------UGG- -5' |
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7148 | 5' | -57.6 | NC_001895.1 | + | 12999 | 0.77 | 0.080891 |
Target: 5'- cGGCGgaauaaCCAGCUCGGCGAUAAUAUa -3' miRNA: 3'- uCCGCgc----GGUCGAGCCGCUGUUAUGg -5' |
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7148 | 5' | -57.6 | NC_001895.1 | + | 5753 | 0.88 | 0.012215 |
Target: 5'- cAGGUGCGCCAGCgcacCGGCGGCAucACCa -3' miRNA: 3'- -UCCGCGCGGUCGa---GCCGCUGUuaUGG- -5' |
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7148 | 5' | -57.6 | NC_001895.1 | + | 20550 | 1.12 | 0.000175 |
Target: 5'- cAGGCGCGCCAGCUCGGCGACAAUACCg -3' miRNA: 3'- -UCCGCGCGGUCGAGCCGCUGUUAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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