Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7149 | 3' | -60.8 | NC_001895.1 | + | 11560 | 0.66 | 0.270464 |
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Target: 5'- aGGCCAGCaUCA-CCGC--CCGUGCg-- -3' miRNA: 3'- -CCGGUCG-AGUcGGCGacGGCACGacu -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 1087 | 0.67 | 0.254183 |
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Target: 5'- cGGUCAGCUUuaccggucuGGCaaaaagccugcggGCUGCCGUGCa-- -3' miRNA: 3'- -CCGGUCGAG---------UCGg------------CGACGGCACGacu -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 20377 | 0.67 | 0.243782 |
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Target: 5'- uGGCCGGaaauauggcCUCAGUgggCGCUGCCgGUGUgggGAu -3' miRNA: 3'- -CCGGUC---------GAGUCG---GCGACGG-CACGa--CU- -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 20103 | 0.67 | 0.243782 |
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Target: 5'- uGGCaagGGCuUCGGCCucCUGCCGUGCc-- -3' miRNA: 3'- -CCGg--UCG-AGUCGGc-GACGGCACGacu -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 442 | 0.69 | 0.186589 |
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Target: 5'- aGCCAGC---GCCGCUGaCC-UGCUGGa -3' miRNA: 3'- cCGGUCGaguCGGCGAC-GGcACGACU- -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 973 | 0.69 | 0.186589 |
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Target: 5'- -cCCAGCaggauuUCAGCCGCU-UUGUGCUGGa -3' miRNA: 3'- ccGGUCG------AGUCGGCGAcGGCACGACU- -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 20241 | 0.69 | 0.176672 |
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Target: 5'- cGGCCAGUUC--CCGCUGCUG-GCg-- -3' miRNA: 3'- -CCGGUCGAGucGGCGACGGCaCGacu -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 22152 | 0.69 | 0.176672 |
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Target: 5'- --gCGGC-CAGCCGgUGCCGUgaccgGCUGAu -3' miRNA: 3'- ccgGUCGaGUCGGCgACGGCA-----CGACU- -5' |
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| 7149 | 3' | -60.8 | NC_001895.1 | + | 21153 | 1.1 | 0.000101 |
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Target: 5'- aGGCCAGCUCAGCCGCUGCCGUGCUGAu -3' miRNA: 3'- -CCGGUCGAGUCGGCGACGGCACGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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