Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7151 | 5' | -52.9 | NC_001895.1 | + | 8559 | 0.66 | 0.703134 |
Target: 5'- uGGCGGCG-UCGCUGUCAUU---CGAa -3' miRNA: 3'- gCUGCCGCuGGCGGUAGUAAaacGCU- -5' |
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7151 | 5' | -52.9 | NC_001895.1 | + | 12269 | 0.67 | 0.676967 |
Target: 5'- gCGGCGGCGACCucaagcggugaugaGCCGgucggCAgcagucGCGAa -3' miRNA: 3'- -GCUGCCGCUGG--------------CGGUa----GUaaaa--CGCU- -5' |
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7151 | 5' | -52.9 | NC_001895.1 | + | 6683 | 0.67 | 0.64825 |
Target: 5'- uCGACGGCGgugccguguggcagcACCGCCcccaGUCAgccagucccggaUUUGCGGc -3' miRNA: 3'- -GCUGCCGC---------------UGGCGG----UAGUa-----------AAACGCU- -5' |
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7151 | 5' | -52.9 | NC_001895.1 | + | 149 | 0.67 | 0.645946 |
Target: 5'- uGACGGgGuguaaaaaaGCCGCCcgCAggcgGCGAu -3' miRNA: 3'- gCUGCCgC---------UGGCGGuaGUaaaaCGCU- -5' |
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7151 | 5' | -52.9 | NC_001895.1 | + | 6333 | 0.68 | 0.56445 |
Target: 5'- gCGAUGGCGACCGCgucgcuuuaugagCGUUAUc--GCGGu -3' miRNA: 3'- -GCUGCCGCUGGCG-------------GUAGUAaaaCGCU- -5' |
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7151 | 5' | -52.9 | NC_001895.1 | + | 21569 | 0.72 | 0.390752 |
Target: 5'- aCGACGGUuggcagugcuguuauGACCGCCAUCGgg--GCu- -3' miRNA: 3'- -GCUGCCG---------------CUGGCGGUAGUaaaaCGcu -5' |
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7151 | 5' | -52.9 | NC_001895.1 | + | 23444 | 0.75 | 0.249652 |
Target: 5'- uGAUGGCGGUCGCCGUCGc--UGCGGg -3' miRNA: 3'- gCUGCCGCUGGCGGUAGUaaaACGCU- -5' |
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7151 | 5' | -52.9 | NC_001895.1 | + | 23393 | 1.09 | 0.000956 |
Target: 5'- gCGACGGCGACCGCCAUCAUUUUGCGAu -3' miRNA: 3'- -GCUGCCGCUGGCGGUAGUAAAACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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