Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7161 | 3' | -59.8 | NC_001900.1 | + | 33348 | 0.66 | 0.425961 |
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Target: 5'- gGGGGAUC-CCAGGgCgaCUCAGUCGg -3' miRNA: 3'- gUUCCUAGaGGUCCgGgaGGGUCGGU- -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 15996 | 0.68 | 0.322207 |
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Target: 5'- aGAGGAUC-CCAGGa--UCCgGGCCGa -3' miRNA: 3'- gUUCCUAGaGGUCCgggAGGgUCGGU- -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 41144 | 0.68 | 0.306862 |
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Target: 5'- --cGGA--UCCGGGCUCUCCgGGUCGa -3' miRNA: 3'- guuCCUagAGGUCCGGGAGGgUCGGU- -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 46840 | 1.07 | 0.000367 |
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Target: 5'- uCAAGGAUCUCCAGGCCCUCCCAGCCAc -3' miRNA: 3'- -GUUCCUAGAGGUCCGGGAGGGUCGGU- -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 20792 | 0.75 | 0.094675 |
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Target: 5'- --cGGGUCUCCacGGaGCCCUCCCGGaCCu -3' miRNA: 3'- guuCCUAGAGG--UC-CGGGAGGGUC-GGu -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 25202 | 0.7 | 0.214968 |
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Target: 5'- cCGAGGuugCgguuacgggCCuGGCCUUCCCAGCCc -3' miRNA: 3'- -GUUCCua-Ga--------GGuCCGGGAGGGUCGGu -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 6032 | 0.67 | 0.346285 |
|
Target: 5'- -cGGGAcgCUCCccGGCUgUCCCGGCUg -3' miRNA: 3'- guUCCUa-GAGGu-CCGGgAGGGUCGGu -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 40146 | 0.67 | 0.354593 |
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Target: 5'- uCGAGGAccgcgucCUCCAGGCgCUCaCAGUCGa -3' miRNA: 3'- -GUUCCUa------GAGGUCCGgGAGgGUCGGU- -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 40488 | 0.66 | 0.398199 |
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Target: 5'- cCGAGGGugaUCUUCuGGCCCcggUCCAGCgGa -3' miRNA: 3'- -GUUCCU---AGAGGuCCGGGa--GGGUCGgU- -5' |
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| 7161 | 3' | -59.8 | NC_001900.1 | + | 43143 | 0.66 | 0.435469 |
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Target: 5'- aGAGGuugCUCCGGGgC-UCCgAGCCGu -3' miRNA: 3'- gUUCCua-GAGGUCCgGgAGGgUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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