Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7163 | 5' | -58.8 | NC_001900.1 | + | 3964 | 0.66 | 0.528656 |
Target: 5'- -aCGCgGCCGGUCUCaCGGgcaccUCGAa-- -3' miRNA: 3'- gaGCGgCGGCCAGAG-GUCa----AGCUccu -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 9389 | 0.67 | 0.467942 |
Target: 5'- --gGCUGUCgagGGUUUCCGGcUCGGGGAc -3' miRNA: 3'- gagCGGCGG---CCAGAGGUCaAGCUCCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 4366 | 0.67 | 0.448503 |
Target: 5'- --aGCCGCCGG-UUCCAgGUUCGAc-- -3' miRNA: 3'- gagCGGCGGCCaGAGGU-CAAGCUccu -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 9204 | 0.67 | 0.429519 |
Target: 5'- aUCGcCCGagacgagaUGGUCUCgGcGUUCGAGGAc -3' miRNA: 3'- gAGC-GGCg-------GCCAGAGgU-CAAGCUCCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 44838 | 0.68 | 0.411016 |
Target: 5'- uUCGCCGUugccggucaacgCGGUCUCCAccgUCGAGc- -3' miRNA: 3'- gAGCGGCG------------GCCAGAGGUca-AGCUCcu -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 31897 | 0.68 | 0.39213 |
Target: 5'- uCUCGacggaaCCGCuCGGUCUCCucggcggucauguAGUgaagUCGAGGAc -3' miRNA: 3'- -GAGC------GGCG-GCCAGAGG-------------UCA----AGCUCCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 35222 | 0.68 | 0.38421 |
Target: 5'- --gGCUGguaCCGGgCUCCGGUaUCGAGGAg -3' miRNA: 3'- gagCGGC---GGCCaGAGGUCA-AGCUCCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 28124 | 0.68 | 0.375536 |
Target: 5'- cCUUGUCGaguuGUCguUCCAGUUCGAGGAu -3' miRNA: 3'- -GAGCGGCggc-CAG--AGGUCAAGCUCCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 1582 | 0.69 | 0.350318 |
Target: 5'- aCUCGg-GCCGGaUCUCCucgcuGUUCGAGGu -3' miRNA: 3'- -GAGCggCGGCC-AGAGGu----CAAGCUCCu -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 45793 | 0.69 | 0.329455 |
Target: 5'- gUCGCgGCCGGugaccaggcagagcaUCUCCAGgUCGAacGGGu -3' miRNA: 3'- gAGCGgCGGCC---------------AGAGGUCaAGCU--CCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 24829 | 0.7 | 0.303579 |
Target: 5'- aUCGCUGCCGGUggugCUGGaggcaaUUCGAGGAa -3' miRNA: 3'- gAGCGGCGGCCAga--GGUC------AAGCUCCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 33960 | 0.7 | 0.296271 |
Target: 5'- gCUCGaCCaGCCGGUggCUCCAGUccaggUCGAcGGGu -3' miRNA: 3'- -GAGC-GG-CGGCCA--GAGGUCA-----AGCU-CCU- -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 8601 | 0.7 | 0.296271 |
Target: 5'- cCUCGCUGaCGGUUUCCGcgUCGGGGu -3' miRNA: 3'- -GAGCGGCgGCCAGAGGUcaAGCUCCu -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 7303 | 0.71 | 0.261784 |
Target: 5'- cCUCGCCGCCGGUCUgggCUuggUCGGGu- -3' miRNA: 3'- -GAGCGGCGGCCAGA---GGucaAGCUCcu -5' |
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7163 | 5' | -58.8 | NC_001900.1 | + | 45696 | 1.09 | 0.00047 |
Target: 5'- gCUCGCCGCCGGUCUCCAGUUCGAGGAa -3' miRNA: 3'- -GAGCGGCGGCCAGAGGUCAAGCUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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