Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7165 | 5' | -56.9 | NC_001900.1 | + | 46491 | 0.66 | 0.609381 |
Target: 5'- -gUUCCCGCCGucGGGCUaCGc-CCUCAg -3' miRNA: 3'- cgAGGGGCGGUu-CCUGA-GCuuGGAGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 44861 | 0.66 | 0.609381 |
Target: 5'- uCUCCaCCGUCGAGcccacgaccuCUCGGAUCUCAu -3' miRNA: 3'- cGAGG-GGCGGUUCcu--------GAGCUUGGAGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 14281 | 0.66 | 0.598454 |
Target: 5'- gGCgUCaCCCGCCGu--GCUCGAACCa-- -3' miRNA: 3'- -CG-AG-GGGCGGUuccUGAGCUUGGagu -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 44298 | 0.66 | 0.598454 |
Target: 5'- gGUUCCCgGUCuuguagucccGGACUCGAAgCUCc -3' miRNA: 3'- -CGAGGGgCGGuu--------CCUGAGCUUgGAGu -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 39128 | 0.66 | 0.597363 |
Target: 5'- cGCUCCCCgaugaccuggacgGCCGGGGGCuuacgcagguagUCGAuCgCUCGc -3' miRNA: 3'- -CGAGGGG-------------CGGUUCCUG------------AGCUuG-GAGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 39170 | 0.66 | 0.58538 |
Target: 5'- aGCUCCgcagUCGCCugcaucagcaucAGGAUUCGAGCCg-- -3' miRNA: 3'- -CGAGG----GGCGGu-----------UCCUGAGCUUGGagu -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 43798 | 0.66 | 0.576694 |
Target: 5'- cGCUCCUCGCUguGAGcGCgagCGAACCg-- -3' miRNA: 3'- -CGAGGGGCGG--UUCcUGa--GCUUGGagu -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 40898 | 0.66 | 0.576694 |
Target: 5'- uGCagCCCGuCCucGGACUCGccAACUUCGc -3' miRNA: 3'- -CGagGGGC-GGuuCCUGAGC--UUGGAGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 42966 | 0.66 | 0.565878 |
Target: 5'- cGC-CUCCGUCGaggGGGACgCGAaGCCUCGc -3' miRNA: 3'- -CGaGGGGCGGU---UCCUGaGCU-UGGAGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 6389 | 0.68 | 0.501315 |
Target: 5'- cCUCCCCGguggaucaggucaCCAGGGGCgUCGAGCaggugCUCGc -3' miRNA: 3'- cGAGGGGC-------------GGUUCCUG-AGCUUG-----GAGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 44500 | 0.68 | 0.461768 |
Target: 5'- -gUCCCUGC--GGGAUUCGAACCcgCGa -3' miRNA: 3'- cgAGGGGCGguUCCUGAGCUUGGa-GU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 30570 | 0.69 | 0.395209 |
Target: 5'- aGCUCagCCGCCAGGGcCUUG-GCCUUc -3' miRNA: 3'- -CGAGg-GGCGGUUCCuGAGCuUGGAGu -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 33000 | 0.7 | 0.363477 |
Target: 5'- cGCUCUucgCCGCCAGGGGCUUccugcgccugcugcgGAGCC-CGa -3' miRNA: 3'- -CGAGG---GGCGGUUCCUGAG---------------CUUGGaGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 3586 | 0.7 | 0.360062 |
Target: 5'- uGUUCCCCGCgu-GGACgcuGAACUUCAa -3' miRNA: 3'- -CGAGGGGCGguuCCUGag-CUUGGAGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 32944 | 0.73 | 0.253542 |
Target: 5'- uGUUCCCCGCCuuggagacaccGGACUUGAACUcCAu -3' miRNA: 3'- -CGAGGGGCGGuu---------CCUGAGCUUGGaGU- -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 3192 | 0.73 | 0.223886 |
Target: 5'- uGCUcCCCCGCCGGGGG-UUGAcCCUCc -3' miRNA: 3'- -CGA-GGGGCGGUUCCUgAGCUuGGAGu -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 44780 | 0.75 | 0.176219 |
Target: 5'- cGCUCuggggaaccgCCCGCUuggcugacgaggAAGGACUCGAACCUUc -3' miRNA: 3'- -CGAG----------GGGCGG------------UUCCUGAGCUUGGAGu -5' |
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7165 | 5' | -56.9 | NC_001900.1 | + | 44604 | 1.1 | 0.000501 |
Target: 5'- uGCUCCCCGCCAAGGACUCGAACCUCAa -3' miRNA: 3'- -CGAGGGGCGGUUCCUGAGCUUGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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