Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7169 | 5' | -49.1 | NC_001900.1 | + | 41556 | 1.07 | 0.004253 |
Target: 5'- uAUUGACGUACUCCGACAGCCAUUUCAa -3' miRNA: 3'- -UAACUGCAUGAGGCUGUCGGUAAAGU- -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 36140 | 0.76 | 0.428196 |
Target: 5'- uGUUGACGUGCUCgGugAGCgAcUUCAc -3' miRNA: 3'- -UAACUGCAUGAGgCugUCGgUaAAGU- -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 41532 | 0.72 | 0.640908 |
Target: 5'- -gUGACGUugUCCGACAGgCCc----- -3' miRNA: 3'- uaACUGCAugAGGCUGUC-GGuaaagu -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 33234 | 0.71 | 0.744474 |
Target: 5'- -cUGGCGUACUUCGGCaccAGCCAc---- -3' miRNA: 3'- uaACUGCAUGAGGCUG---UCGGUaaagu -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 39265 | 0.71 | 0.744474 |
Target: 5'- cUUGugGUcaugguCUaCCGAUAGCCGUUUCc -3' miRNA: 3'- uAACugCAu-----GA-GGCUGUCGGUAAAGu -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 2245 | 0.68 | 0.855917 |
Target: 5'- cUUGA-GUACUCgggUGACGGCCAggUCGa -3' miRNA: 3'- uAACUgCAUGAG---GCUGUCGGUaaAGU- -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 8644 | 0.68 | 0.889285 |
Target: 5'- -cUGcCGgguCUCCGACGGCCugcugUUCGu -3' miRNA: 3'- uaACuGCau-GAGGCUGUCGGua---AAGU- -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 21566 | 0.67 | 0.92305 |
Target: 5'- --aGACgGUGCgccgcuacaucgCCGACGGCCGgcUCAa -3' miRNA: 3'- uaaCUG-CAUGa-----------GGCUGUCGGUaaAGU- -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 12481 | 0.67 | 0.924302 |
Target: 5'- --cGAUGcACggcaCCGACAGCCcgUUCc -3' miRNA: 3'- uaaCUGCaUGa---GGCUGUCGGuaAAGu -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 46777 | 0.66 | 0.946707 |
Target: 5'- -cUGGCGUGUUCCGccuCAGCCAa---- -3' miRNA: 3'- uaACUGCAUGAGGCu--GUCGGUaaagu -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 43670 | 0.66 | 0.930372 |
Target: 5'- -cUGGCGUACUCCG-CAGUgGc---- -3' miRNA: 3'- uaACUGCAUGAGGCuGUCGgUaaagu -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 23057 | 0.66 | 0.930372 |
Target: 5'- ---aACGUACUCCGgGCGGUCAUg--- -3' miRNA: 3'- uaacUGCAUGAGGC-UGUCGGUAaagu -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 23810 | 0.66 | 0.941573 |
Target: 5'- --cGACGgGCUCCGGuCGGCUA--UCGg -3' miRNA: 3'- uaaCUGCaUGAGGCU-GUCGGUaaAGU- -5' |
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7169 | 5' | -49.1 | NC_001900.1 | + | 36339 | 0.66 | 0.946707 |
Target: 5'- cUUGACGUugUCCGACgAGUa------ -3' miRNA: 3'- uAACUGCAugAGGCUG-UCGguaaagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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