Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 717 | 5' | -52.8 | AC_000019.1 | + | 10709 | 0.66 | 0.702473 |
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Target: 5'- -uUGGGGGCGCAucuguCGCAGcACGggaUGCa -3' miRNA: 3'- auGCCUCCGCGU-----GCGUU-UGCaagAUG- -5' |
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| 717 | 5' | -52.8 | AC_000019.1 | + | 19272 | 0.66 | 0.690962 |
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Target: 5'- cAUGGGGGUGCugGCuggucAAGCGUcUCa-- -3' miRNA: 3'- aUGCCUCCGCGugCG-----UUUGCA-AGaug -5' |
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| 717 | 5' | -52.8 | AC_000019.1 | + | 8603 | 0.68 | 0.540007 |
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Target: 5'- -gUGGuGGCGCACGCAAGucUUCUcagaGCg -3' miRNA: 3'- auGCCuCCGCGUGCGUUUgcAAGA----UG- -5' |
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| 717 | 5' | -52.8 | AC_000019.1 | + | 9333 | 0.69 | 0.521934 |
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Target: 5'- gGCGGcGGCGCaugguuucagugacgGCGCGGcCGUUCUcGCg -3' miRNA: 3'- aUGCCuCCGCG---------------UGCGUUuGCAAGA-UG- -5' |
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| 717 | 5' | -52.8 | AC_000019.1 | + | 10353 | 0.69 | 0.495282 |
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Target: 5'- cACGGuuugaccagugAGGCGCGCGCAgucauuGAUGcUCUAUa -3' miRNA: 3'- aUGCC-----------UCCGCGUGCGU------UUGCaAGAUG- -5' |
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| 717 | 5' | -52.8 | AC_000019.1 | + | 9260 | 0.7 | 0.452315 |
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Target: 5'- gGCGGAgGGgGCACGCGgcGACG-UCgacgGCg -3' miRNA: 3'- aUGCCU-CCgCGUGCGU--UUGCaAGa---UG- -5' |
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| 717 | 5' | -52.8 | AC_000019.1 | + | 13612 | 0.71 | 0.421463 |
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Target: 5'- aGCGaGGGCGCAaaugaGCAAACGggUUGCc -3' miRNA: 3'- aUGCcUCCGCGUg----CGUUUGCaaGAUG- -5' |
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| 717 | 5' | -52.8 | AC_000019.1 | + | 15432 | 1.07 | 0.001142 |
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Target: 5'- gUACGGAGGCGCACGCAAACGUUCUACc -3' miRNA: 3'- -AUGCCUCCGCGUGCGUUUGCAAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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