Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7171 | 5' | -63.8 | NC_001900.1 | + | 21202 | 0.66 | 0.308714 |
Target: 5'- cGGCCCUGGUgaacaUCGGUGGcgaucugcugAccucguucaucaacaGCCAGCUCGc -3' miRNA: 3'- -CCGGGGCCA-----GGUCGCC----------U---------------CGGUCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 18349 | 0.66 | 0.298676 |
Target: 5'- -cCCCCGG-CCuuuCGGGGCCGGUggguUCGg -3' miRNA: 3'- ccGGGGCCaGGuc-GCCUCGGUCG----AGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 30721 | 0.66 | 0.298676 |
Target: 5'- cGGCCCCGaaaggCCGGgGGuccaCCAGCUUc -3' miRNA: 3'- -CCGGGGCca---GGUCgCCuc--GGUCGAGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 9784 | 0.66 | 0.298676 |
Target: 5'- cGGUCCCGGuggucccgcuUCCGaacCGGAcCCGGCUCu -3' miRNA: 3'- -CCGGGGCC----------AGGUc--GCCUcGGUCGAGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 38893 | 0.66 | 0.291662 |
Target: 5'- cGGCCCUgauGGcCuCAGCGGAGgCCGGaugUCa -3' miRNA: 3'- -CCGGGG---CCaG-GUCGCCUC-GGUCg--AGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 43468 | 0.66 | 0.291662 |
Target: 5'- -cCCCCGcGUCauggauCGGccGGCCAGCUCGg -3' miRNA: 3'- ccGGGGC-CAGguc---GCC--UCGGUCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 25759 | 0.66 | 0.284778 |
Target: 5'- cGCCCCGG-CgCAcGCGGAGCUucaUCGu -3' miRNA: 3'- cCGGGGCCaG-GU-CGCCUCGGucgAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 37271 | 0.67 | 0.264896 |
Target: 5'- gGGCCgucguacaCGGcCCgcAGCGGGGCCAGgaacCUCGu -3' miRNA: 3'- -CCGGg-------GCCaGG--UCGCCUCGGUC----GAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 6476 | 0.67 | 0.258524 |
Target: 5'- aGGCUCUGGagUCCGcugucaacggcGCGGucccCCAGCUCGg -3' miRNA: 3'- -CCGGGGCC--AGGU-----------CGCCuc--GGUCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 41235 | 0.67 | 0.246155 |
Target: 5'- cGGCCgcgaCGGugaUCCAcgcuGCGGGGCgCGGCUUGc -3' miRNA: 3'- -CCGGg---GCC---AGGU----CGCCUCG-GUCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 47883 | 0.67 | 0.23428 |
Target: 5'- cGCCCCGGccgcuUCCGGCGGcccaccGCCGcCUCc -3' miRNA: 3'- cCGGGGCC-----AGGUCGCCu-----CGGUcGAGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 17365 | 0.67 | 0.233699 |
Target: 5'- -aCCUgCGGUCgGagaacgccgaucuGCGGAGUCAGCUCGa -3' miRNA: 3'- ccGGG-GCCAGgU-------------CGCCUCGGUCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 46001 | 0.68 | 0.222891 |
Target: 5'- -cCCgCCGGUCggguCGGCGGGuGCCcGCUCGa -3' miRNA: 3'- ccGG-GGCCAG----GUCGCCU-CGGuCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 25820 | 0.68 | 0.211976 |
Target: 5'- uGGUCCUGugcGUCCAG-GcGAGCCugcAGCUCGg -3' miRNA: 3'- -CCGGGGC---CAGGUCgC-CUCGG---UCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 43103 | 0.68 | 0.201524 |
Target: 5'- cGGCCuggCCGGUUCAGC--AGCCAGCcCa -3' miRNA: 3'- -CCGG---GGCCAGGUCGccUCGGUCGaGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 39695 | 0.69 | 0.172376 |
Target: 5'- cGCCgUGGgaacagaUCCAGCaGAGCCAGCgCGa -3' miRNA: 3'- cCGGgGCC-------AGGUCGcCUCGGUCGaGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 10657 | 0.7 | 0.155767 |
Target: 5'- gGGCUcgCCGGguacgUCCAGCGGuucGCCAGuCUCu -3' miRNA: 3'- -CCGG--GGCC-----AGGUCGCCu--CGGUC-GAGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 45043 | 0.7 | 0.155767 |
Target: 5'- cGGCCagaUGGUCaGGCGGuccuuGCCuGGCUCGa -3' miRNA: 3'- -CCGGg--GCCAGgUCGCCu----CGG-UCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 30482 | 0.7 | 0.147823 |
Target: 5'- cGGCCggcucacgUCGGgcaCCAGCGGuGCCAGCg-- -3' miRNA: 3'- -CCGG--------GGCCa--GGUCGCCuCGGUCGagc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 20235 | 0.7 | 0.143991 |
Target: 5'- uGGCUCCGGUCUGGUucgGGGGCCAcgUCa -3' miRNA: 3'- -CCGGGGCCAGGUCG---CCUCGGUcgAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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