Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7171 | 5' | -63.8 | NC_001900.1 | + | 43468 | 0.66 | 0.291662 |
Target: 5'- -cCCCCGcGUCauggauCGGccGGCCAGCUCGg -3' miRNA: 3'- ccGGGGC-CAGguc---GCC--UCGGUCGAGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 30721 | 0.66 | 0.298676 |
Target: 5'- cGGCCCCGaaaggCCGGgGGuccaCCAGCUUc -3' miRNA: 3'- -CCGGGGCca---GGUCgCCuc--GGUCGAGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 9784 | 0.66 | 0.298676 |
Target: 5'- cGGUCCCGGuggucccgcuUCCGaacCGGAcCCGGCUCu -3' miRNA: 3'- -CCGGGGCC----------AGGUc--GCCUcGGUCGAGc -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 18349 | 0.66 | 0.298676 |
Target: 5'- -cCCCCGG-CCuuuCGGGGCCGGUggguUCGg -3' miRNA: 3'- ccGGGGCCaGGuc-GCCUCGGUCG----AGC- -5' |
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7171 | 5' | -63.8 | NC_001900.1 | + | 21202 | 0.66 | 0.308714 |
Target: 5'- cGGCCCUGGUgaacaUCGGUGGcgaucugcugAccucguucaucaacaGCCAGCUCGc -3' miRNA: 3'- -CCGGGGCCA-----GGUCGCC----------U---------------CGGUCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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