Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7172 | 5' | -57 | NC_001900.1 | + | 27193 | 0.66 | 0.6048 |
Target: 5'- -aCGACGUGCuGUCGcGGGUcGUUCGGa -3' miRNA: 3'- caGCUGCAUGuCGGCcCCCA-UAGGCU- -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 8215 | 0.66 | 0.6048 |
Target: 5'- --gGACGUGCAGuCCGGGaa-GUCCGu -3' miRNA: 3'- cagCUGCAUGUC-GGCCCccaUAGGCu -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 35961 | 0.66 | 0.583136 |
Target: 5'- cUCGuCGcGCAGCCcGGGGUGcUCGAc -3' miRNA: 3'- cAGCuGCaUGUCGGcCCCCAUaGGCU- -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 3131 | 0.67 | 0.561632 |
Target: 5'- --aGGCGaGCGGCgGGGGGUgaugGUCCc- -3' miRNA: 3'- cagCUGCaUGUCGgCCCCCA----UAGGcu -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 3187 | 0.67 | 0.529809 |
Target: 5'- -cCGAC-UGCucccccGCCGGGGGUugacccUCCGAa -3' miRNA: 3'- caGCUGcAUGu-----CGGCCCCCAu-----AGGCU- -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 27315 | 0.68 | 0.467381 |
Target: 5'- --gGAUGUGCagAGCUGGGuucuucgguucgcGGUAUCCGAu -3' miRNA: 3'- cagCUGCAUG--UCGGCCC-------------CCAUAGGCU- -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 38547 | 0.68 | 0.458487 |
Target: 5'- -gCGACcguguuGUACAGCCGGGGcuUAUCCc- -3' miRNA: 3'- caGCUG------CAUGUCGGCCCCc-AUAGGcu -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 28258 | 0.69 | 0.410806 |
Target: 5'- aGUCGACGgauCAGguCCGGGaGGgcUCCGu -3' miRNA: 3'- -CAGCUGCau-GUC--GGCCC-CCauAGGCu -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 39472 | 0.7 | 0.401645 |
Target: 5'- cUCGGCGUACAugcgcGUCGGGGGcuccacCCGGa -3' miRNA: 3'- cAGCUGCAUGU-----CGGCCCCCaua---GGCU- -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 23179 | 0.71 | 0.32528 |
Target: 5'- --gGACGUAgUGGCCGGGgaGGUGUUCGAg -3' miRNA: 3'- cagCUGCAU-GUCGGCCC--CCAUAGGCU- -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 47590 | 0.76 | 0.166599 |
Target: 5'- -gCGACGUuucguCGGCCGGGGGUGg-CGAa -3' miRNA: 3'- caGCUGCAu----GUCGGCCCCCAUagGCU- -5' |
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7172 | 5' | -57 | NC_001900.1 | + | 39717 | 1.08 | 0.00074 |
Target: 5'- aGUCGACGUACAGCCGGGGGUAUCCGAc -3' miRNA: 3'- -CAGCUGCAUGUCGGCCCCCAUAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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