Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7175 | 3' | -50.9 | NC_001900.1 | + | 3954 | 0.71 | 0.653091 |
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Target: 5'- uCCACGGcGAAcgCGGCcgGUCucacGGGCACCu -3' miRNA: 3'- -GGUGCC-CUUuaGUCG--UAG----UCUGUGGu -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 19816 | 0.72 | 0.596037 |
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Target: 5'- uCUACGGcGAGAaCuGCAUCAucGACACCGa -3' miRNA: 3'- -GGUGCC-CUUUaGuCGUAGU--CUGUGGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 31118 | 0.73 | 0.528737 |
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Target: 5'- aUCGCGGG-GAUCAGCGUCuGGaACGCUg -3' miRNA: 3'- -GGUGCCCuUUAGUCGUAG-UC-UGUGGu -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 29016 | 0.76 | 0.376361 |
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Target: 5'- aCCuuCGGGGAcAUCGGCGgugUAGACGCCGa -3' miRNA: 3'- -GGu-GCCCUU-UAGUCGUa--GUCUGUGGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 5911 | 0.77 | 0.340996 |
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Target: 5'- uCCugGGGGAcAUCAGC-UCAGGCGgCAc -3' miRNA: 3'- -GGugCCCUU-UAGUCGuAGUCUGUgGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 14071 | 0.77 | 0.31609 |
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Target: 5'- gCACGGGAuccUCGGCGcUCAGAC-CCAg -3' miRNA: 3'- gGUGCCCUuu-AGUCGU-AGUCUGuGGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 37980 | 1.11 | 0.001708 |
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Target: 5'- gCCACGGGAAAUCAGCAUCAGACACCAg -3' miRNA: 3'- -GGUGCCCUUUAGUCGUAGUCUGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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