Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7175 | 5' | -62.3 | NC_001900.1 | + | 48701 | 0.69 | 0.205802 |
Target: 5'- -gUCGuCGCCgGGUGGCuGuCCagUCGCCc -3' miRNA: 3'- aaAGC-GCGGgCCACCG-CuGGg-AGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 47882 | 0.68 | 0.233683 |
Target: 5'- --cCGC-CCCGGccgcuuccGGCGGCCCacCGCCg -3' miRNA: 3'- aaaGCGcGGGCCa-------CCGCUGGGa-GCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 47309 | 0.73 | 0.100374 |
Target: 5'- -gUCGCGUCCGGgcaGGagaACCCUUGCCc -3' miRNA: 3'- aaAGCGCGGGCCa--CCgc-UGGGAGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 38014 | 1.08 | 0.000234 |
Target: 5'- gUUUCGCGCCCGGUGGCGACCCUCGCCc -3' miRNA: 3'- -AAAGCGCGGGCCACCGCUGGGAGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 36145 | 0.72 | 0.135203 |
Target: 5'- --aCGUGCUCGGUGaGCGACUucacggCUCGCUu -3' miRNA: 3'- aaaGCGCGGGCCAC-CGCUGG------GAGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 36009 | 0.69 | 0.198528 |
Target: 5'- --cCGCGugaacggcccccuCCCGGUGGaUGucaaguuaggcucaGCCCUCGCCg -3' miRNA: 3'- aaaGCGC-------------GGGCCACC-GC--------------UGGGAGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 34843 | 0.69 | 0.227863 |
Target: 5'- --aCGUGCCUGGccGCGACCUuguUCGCg -3' miRNA: 3'- aaaGCGCGGGCCacCGCUGGG---AGCGg -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 32234 | 0.68 | 0.251893 |
Target: 5'- --cCGCcacGCCCGGUGGUuGCCCgugaCGaCCa -3' miRNA: 3'- aaaGCG---CGGGCCACCGcUGGGa---GC-GG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 26257 | 0.7 | 0.180375 |
Target: 5'- -cUCGCGCUCGGccGGCGGgguccacUCCUCGUUg -3' miRNA: 3'- aaAGCGCGGGCCa-CCGCU-------GGGAGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 25170 | 0.68 | 0.245697 |
Target: 5'- -cUCGgGCCgGGUacgaGGCGAgCCU-GCCg -3' miRNA: 3'- aaAGCgCGGgCCA----CCGCUgGGAgCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 23318 | 0.69 | 0.227863 |
Target: 5'- --gUGCGCCgGG-GcGCGGCCCgCGUCg -3' miRNA: 3'- aaaGCGCGGgCCaC-CGCUGGGaGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 21268 | 0.69 | 0.200583 |
Target: 5'- --gCGCGgUCGGUGGCGcgaucgaccuGCCCcCGCUg -3' miRNA: 3'- aaaGCGCgGGCCACCGC----------UGGGaGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 21079 | 0.66 | 0.344397 |
Target: 5'- cUUCGUGCCCGccgggGGCGGCgUcaacgUGCCg -3' miRNA: 3'- aAAGCGCGGGCca---CCGCUGgGa----GCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 21006 | 0.66 | 0.343599 |
Target: 5'- cUUUCGCccuggGCCucacggccugugaCGGUGGCGGCUC-CGCa -3' miRNA: 3'- -AAAGCG-----CGG-------------GCCACCGCUGGGaGCGg -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 18357 | 0.74 | 0.097665 |
Target: 5'- cUUUCGgGgCCGGUGGguuCGGCCaCUCGCUg -3' miRNA: 3'- -AAAGCgCgGGCCACC---GCUGG-GAGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 17107 | 0.66 | 0.344397 |
Target: 5'- ---aGCaGCUCGGUGGguugcugaCGACCCUCaCCc -3' miRNA: 3'- aaagCG-CGGGCCACC--------GCUGGGAGcGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 9136 | 0.68 | 0.239627 |
Target: 5'- --aCGgGCCgGGUGGUGGCCgUCcgaugacaGCCc -3' miRNA: 3'- aaaGCgCGGgCCACCGCUGGgAG--------CGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 4986 | 0.67 | 0.284819 |
Target: 5'- -aUCGCGCCuCGaUGuuCGACCCgUUGCCg -3' miRNA: 3'- aaAGCGCGG-GCcACc-GCUGGG-AGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 4150 | 0.65 | 0.358158 |
Target: 5'- ---aGCGgCCGGcGGUGACCCcaaacaacucggcgUCGUCu -3' miRNA: 3'- aaagCGCgGGCCaCCGCUGGG--------------AGCGG- -5' |
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7175 | 5' | -62.3 | NC_001900.1 | + | 1616 | 0.66 | 0.313538 |
Target: 5'- cUUCuCGgCCGGUGGCGAcaccCCCggucagCGCg -3' miRNA: 3'- aAAGcGCgGGCCACCGCU----GGGa-----GCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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