Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7176 | 3' | -57.9 | NC_001900.1 | + | 17467 | 0.66 | 0.563541 |
Target: 5'- --aCCgGCCGaACCGCCaGAAGCUCa-- -3' miRNA: 3'- caaGGaCGGC-UGGUGG-CUUCGGGacc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 7463 | 0.66 | 0.563541 |
Target: 5'- -aUCgaGcCCGA-CACCGGAGCCCagaagcUGGa -3' miRNA: 3'- caAGgaC-GGCUgGUGGCUUCGGG------ACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 20715 | 0.66 | 0.542251 |
Target: 5'- cUUCgCUGUCGACCagACCGAcggcagcaAGCUgUGGc -3' miRNA: 3'- cAAG-GACGGCUGG--UGGCU--------UCGGgACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 13921 | 0.66 | 0.530655 |
Target: 5'- aUUCCUcGCUGACUACCcgcacguGAAGCaggagCUGGa -3' miRNA: 3'- cAAGGA-CGGCUGGUGG-------CUUCGg----GACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 15082 | 0.66 | 0.521235 |
Target: 5'- --aCCUGuCCGACCcgucgaccuggACUGGAGCCaccggCUGGu -3' miRNA: 3'- caaGGAC-GGCUGG-----------UGGCUUCGG-----GACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 35094 | 0.67 | 0.499518 |
Target: 5'- -gUCgUGCCGACgUACCGGacugguuGGCgaCCUGGa -3' miRNA: 3'- caAGgACGGCUG-GUGGCU-------UCG--GGACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 16623 | 0.67 | 0.490333 |
Target: 5'- cGUUCCggucaacGCCGACCugaaCGcGGCCCaGGc -3' miRNA: 3'- -CAAGGa------CGGCUGGug--GCuUCGGGaCC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 25759 | 0.67 | 0.480221 |
Target: 5'- --gCCgggGCCGACCGCCGuGGUgUUGa -3' miRNA: 3'- caaGGa--CGGCUGGUGGCuUCGgGACc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 25127 | 0.67 | 0.460311 |
Target: 5'- --aCCgaGCCG-CCACCGAGaCCgCUGGg -3' miRNA: 3'- caaGGa-CGGCuGGUGGCUUcGG-GACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 18474 | 0.68 | 0.431291 |
Target: 5'- gGUUCCaGCCcgUCAUCGAgaaggccaaGGCCCUGGc -3' miRNA: 3'- -CAAGGaCGGcuGGUGGCU---------UCGGGACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 33050 | 0.68 | 0.421858 |
Target: 5'- -gUgCUGCUGGCCACCGccGCCgCUa- -3' miRNA: 3'- caAgGACGGCUGGUGGCuuCGG-GAcc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 43527 | 0.68 | 0.421858 |
Target: 5'- --cCCUGCCGugagcCCACCGGgagaAGUCCcGGu -3' miRNA: 3'- caaGGACGGCu----GGUGGCU----UCGGGaCC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 3154 | 0.68 | 0.421858 |
Target: 5'- -aUCCU-CgCGGCCAUCGAAGUCUUGa -3' miRNA: 3'- caAGGAcG-GCUGGUGGCUUCGGGACc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 3984 | 0.68 | 0.412551 |
Target: 5'- --cCCUGCUG-CCgcguACCGGAGCCCg-- -3' miRNA: 3'- caaGGACGGCuGG----UGGCUUCGGGacc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 46703 | 0.68 | 0.412551 |
Target: 5'- aGUUCUUGCCGACgCuuCGAgccGGCCCa-- -3' miRNA: 3'- -CAAGGACGGCUG-GugGCU---UCGGGacc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 13139 | 0.69 | 0.394322 |
Target: 5'- --gCC-GCCGAUC-CCGGAGuUCCUGGg -3' miRNA: 3'- caaGGaCGGCUGGuGGCUUC-GGGACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 48147 | 0.69 | 0.376622 |
Target: 5'- aGUUCCUGgaGGCCAgCGcggaaAAGCgCCUGGu -3' miRNA: 3'- -CAAGGACggCUGGUgGC-----UUCG-GGACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 29737 | 0.69 | 0.359466 |
Target: 5'- --gUCUGCuCGGCCACCGuguuGGCCCc-- -3' miRNA: 3'- caaGGACG-GCUGGUGGCu---UCGGGacc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 21720 | 0.69 | 0.351095 |
Target: 5'- cUUCgUGCUGGCCcuuCUGGAGCCCUu- -3' miRNA: 3'- cAAGgACGGCUGGu--GGCUUCGGGAcc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 4129 | 0.7 | 0.334772 |
Target: 5'- gGUUCauCUGCCGACCGgCGAAGCggCCggcGGu -3' miRNA: 3'- -CAAG--GACGGCUGGUgGCUUCG--GGa--CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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