Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7177 | 3' | -57.4 | NC_001900.1 | + | 35341 | 0.66 | 0.5678 |
Target: 5'- gGAUCCGCuucgUGGucGCACaUCGCGgCGUCGAc -3' miRNA: 3'- -CUAGGUG----ACC--CGUG-AGUGCaGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 47335 | 0.66 | 0.5678 |
Target: 5'- --cCCACUGGGCAgaccgaugcugUUCGCGaUGCCu- -3' miRNA: 3'- cuaGGUGACCCGU-----------GAGUGCaGCGGcu -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 36720 | 0.66 | 0.564564 |
Target: 5'- cGAUCUugUgGGGCGCgauggucugcgaagUCAUGUUGCCc- -3' miRNA: 3'- -CUAGGugA-CCCGUG--------------AGUGCAGCGGcu -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 17606 | 0.66 | 0.546328 |
Target: 5'- cGUCCucgggacuCUGGGCACUCAgcUCGCucCGAu -3' miRNA: 3'- cUAGGu-------GACCCGUGAGUgcAGCG--GCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 22945 | 0.67 | 0.525122 |
Target: 5'- gGAUCUACccguguUGGGCGa-CACcccgGUCGCCGAg -3' miRNA: 3'- -CUAGGUG------ACCCGUgaGUG----CAGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 35426 | 0.67 | 0.504236 |
Target: 5'- --cCCGCUGaacgucgcuGGCGCUCAUGUCGgCa- -3' miRNA: 3'- cuaGGUGAC---------CCGUGAGUGCAGCgGcu -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 5854 | 0.67 | 0.483718 |
Target: 5'- aGAUCCACgGGGCGCUguucaaCAcCGUCGUg-- -3' miRNA: 3'- -CUAGGUGaCCCGUGA------GU-GCAGCGgcu -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 15009 | 0.67 | 0.483718 |
Target: 5'- --gCUGCggucGGGUAUgugUACGUCGCCGAa -3' miRNA: 3'- cuaGGUGa---CCCGUGa--GUGCAGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 37368 | 0.67 | 0.469596 |
Target: 5'- uGGUCCACcgagacGGGCucggguaguccaUCAgGUCGCCGAu -3' miRNA: 3'- -CUAGGUGa-----CCCGug----------AGUgCAGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 10510 | 0.68 | 0.463609 |
Target: 5'- --cCUGCUGGGCACgaugguCGaCGCCGAc -3' miRNA: 3'- cuaGGUGACCCGUGagu---GCaGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 46252 | 0.68 | 0.42476 |
Target: 5'- cGAUgaGCggaUGGGUcucgcccauguaGCUCAUGUCGCCGAg -3' miRNA: 3'- -CUAggUG---ACCCG------------UGAGUGCAGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 40357 | 0.68 | 0.42476 |
Target: 5'- cGUCCACgaacucgcgGGGCACUU-CGcCGCCGu -3' miRNA: 3'- cUAGGUGa--------CCCGUGAGuGCaGCGGCu -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 41247 | 0.69 | 0.387921 |
Target: 5'- uGAUCCACgcugcgGGGCGCg-GC-UUGCCGAu -3' miRNA: 3'- -CUAGGUGa-----CCCGUGagUGcAGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 19657 | 0.69 | 0.370305 |
Target: 5'- --gCCAacugGGGCGCUCGCGagaUCGCuCGAc -3' miRNA: 3'- cuaGGUga--CCCGUGAGUGC---AGCG-GCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 23312 | 0.71 | 0.305461 |
Target: 5'- -cUCCGCgugcgccgGGGCGCggccCGCGUCGgCGAg -3' miRNA: 3'- cuAGGUGa-------CCCGUGa---GUGCAGCgGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 9479 | 0.72 | 0.249687 |
Target: 5'- --cCCGCUGGGCuacaccgacGCUUACGUCcacgGCCGGu -3' miRNA: 3'- cuaGGUGACCCG---------UGAGUGCAG----CGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 8310 | 0.74 | 0.18702 |
Target: 5'- uGGUUCACgGcGGCGCUCGCGgCGCUGAc -3' miRNA: 3'- -CUAGGUGaC-CCGUGAGUGCaGCGGCU- -5' |
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7177 | 3' | -57.4 | NC_001900.1 | + | 36789 | 1.08 | 0.000588 |
Target: 5'- cGAUCCACUGGGCACUCACGUCGCCGAc -3' miRNA: 3'- -CUAGGUGACCCGUGAGUGCAGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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