Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7177 | 5' | -57.9 | NC_001900.1 | + | 17716 | 0.65 | 0.555734 |
Target: 5'- -cUCUGGggccugugcugaCCGUGGUcgcugagACCC-UCGGCAc -3' miRNA: 3'- gaAGACCg-----------GGCACCA-------UGGGuAGCCGU- -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 24162 | 0.66 | 0.516533 |
Target: 5'- -gUCguaGGUCCGgucucGGUACaCCAugUCGGCAg -3' miRNA: 3'- gaAGa--CCGGGCa----CCAUG-GGU--AGCCGU- -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 42447 | 0.67 | 0.4795 |
Target: 5'- --gCUGGCCCGUgccgugaacGGUgaacagccagggcuuGCUCAUCGGUu -3' miRNA: 3'- gaaGACCGGGCA---------CCA---------------UGGGUAGCCGu -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 21198 | 0.67 | 0.475464 |
Target: 5'- -aUCUcGGCCC-UGGUGaaCAUCGGUg -3' miRNA: 3'- gaAGA-CCGGGcACCAUggGUAGCCGu -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 6696 | 0.68 | 0.436088 |
Target: 5'- -aUCUa-CCgGUGGcaGCCCAUCGGCAa -3' miRNA: 3'- gaAGAccGGgCACCa-UGGGUAGCCGU- -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 18574 | 0.68 | 0.417117 |
Target: 5'- --gCUGGCaCCGcUGGUGCCCGacgugagcCGGCc -3' miRNA: 3'- gaaGACCG-GGC-ACCAUGGGUa-------GCCGu -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 25015 | 0.68 | 0.389628 |
Target: 5'- -gUUUGGCCgGUGGUcccgguGCCCGUggccaGGCGu -3' miRNA: 3'- gaAGACCGGgCACCA------UGGGUAg----CCGU- -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 35941 | 0.69 | 0.380732 |
Target: 5'- -aUCggcGGCUCGUagaGGUACUCGUCGcGCAg -3' miRNA: 3'- gaAGa--CCGGGCA---CCAUGGGUAGC-CGU- -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 23792 | 0.69 | 0.377212 |
Target: 5'- --aCUGGCCCuGUGGUccgacgacgggcuCCgGUCGGCu -3' miRNA: 3'- gaaGACCGGG-CACCAu------------GGgUAGCCGu -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 43865 | 0.7 | 0.299465 |
Target: 5'- --gCUGGgCCGacUGGUGCCacuUCGGCAu -3' miRNA: 3'- gaaGACCgGGC--ACCAUGGgu-AGCCGU- -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 33867 | 0.71 | 0.270929 |
Target: 5'- --aCUGGUCCuucucgacGUGGUACCCGUCaGCc -3' miRNA: 3'- gaaGACCGGG--------CACCAUGGGUAGcCGu -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 33163 | 0.73 | 0.203718 |
Target: 5'- aCUUCUGGCCC-UGG--CCCAgcUUGGCAa -3' miRNA: 3'- -GAAGACCGGGcACCauGGGU--AGCCGU- -5' |
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7177 | 5' | -57.9 | NC_001900.1 | + | 36828 | 1.08 | 0.000576 |
Target: 5'- uCUUCUGGCCCGUGGUACCCAUCGGCAc -3' miRNA: 3'- -GAAGACCGGGCACCAUGGGUAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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