Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7178 | 5' | -56.6 | NC_001900.1 | + | 30004 | 0.66 | 0.670356 |
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Target: 5'- aGUCGgACGCGGgcagCGUcuGCGCCGGg-- -3' miRNA: 3'- -CAGCaUGUGCCa---GCAc-CGUGGCCacc -5' |
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| 7178 | 5' | -56.6 | NC_001900.1 | + | 30073 | 0.66 | 0.659557 |
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Target: 5'- --gGUugACGaacgUGUGGCACCGGUccucGGa -3' miRNA: 3'- cagCAugUGCca--GCACCGUGGCCA----CC- -5' |
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| 7178 | 5' | -56.6 | NC_001900.1 | + | 6548 | 0.66 | 0.648733 |
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Target: 5'- cUUGUauGCGcCGGUCcUGGCACUGGUa- -3' miRNA: 3'- cAGCA--UGU-GCCAGcACCGUGGCCAcc -5' |
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| 7178 | 5' | -56.6 | NC_001900.1 | + | 33935 | 0.66 | 0.625966 |
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Target: 5'- -gCGUGCcuCGGcuggUGUGGCcgacgcucgaccaGCCGGUGGc -3' miRNA: 3'- caGCAUGu-GCCa---GCACCG-------------UGGCCACC- -5' |
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| 7178 | 5' | -56.6 | NC_001900.1 | + | 23735 | 0.67 | 0.605384 |
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Target: 5'- gGUCGcugGCACGGUCGacGGCAUCGa--- -3' miRNA: 3'- -CAGCa--UGUGCCAGCa-CCGUGGCcacc -5' |
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| 7178 | 5' | -56.6 | NC_001900.1 | + | 24694 | 0.69 | 0.493539 |
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Target: 5'- -cCGUGgGCGGUcacacagaucacuggCGUcaucGGCGCUGGUGGu -3' miRNA: 3'- caGCAUgUGCCA---------------GCA----CCGUGGCCACC- -5' |
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| 7178 | 5' | -56.6 | NC_001900.1 | + | 37227 | 0.69 | 0.459591 |
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Target: 5'- aGUCGaacgGCcgGCGGUCGUGGUugCcuucGUGGa -3' miRNA: 3'- -CAGCa---UG--UGCCAGCACCGugGc---CACC- -5' |
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| 7178 | 5' | -56.6 | NC_001900.1 | + | 36510 | 1.09 | 0.000741 |
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Target: 5'- gGUCGUACACGGUCGUGGCACCGGUGGu -3' miRNA: 3'- -CAGCAUGUGCCAGCACCGUGGCCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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